CAJ | 학술논문

目的检测整合素β1在皮肤鳞状细胞癌组织中的表达情况,探索干细胞相关因子与肿瘤的关系.方法采用免疫组织化学及细胞学染色方法检测整合素β1在人皮肤鳞状细胞癌组织及人皮肤鳞状细胞癌细胞株A431中的表达,全反式维甲酸(ATRA)诱导鳞状细胞癌细胞分化,RT-PCR检测分化前后整合素β1变化水平.结果在高分化鳞状细胞癌中,整合素β1在肿瘤团块外周基底样细胞呈连续性表达,内侧至团块中央可见散在或岛屿状阳性细胞;在低分化鳞状细胞癌组织中,岛屿状阳性细胞明显增多,呈弥漫分布;在A431细胞中,整合素β1散在表达于肿瘤细胞.ATRA诱导24h和48h后,ATRA处理组整合素β1与内参照β-actin的RT-PCR产物电泳光密度值之比分别为0.071±0.025和0.029±0.018,明显低于对照组的0.148±0.027和0.136±0.011(P<0.05).结论整合素β1在皮肤鳞状细胞癌组织培养肿瘤细胞中具有异质性表达模式,并随肿瘤细胞分化程度增高而表达下降,提示整合素β1与皮肤鳞状细胞癌发生及可能存在的肿瘤干细胞具有密切关系.
목적 검측정합소β1재피부린상세포암조직중적표체정황,탐색간세포상관인자여종류적관계.방법 채용면역조직화학급세포학염색방법검측정합소β1재인피부린상세포암조직급인피부린상세포암세포주A431중적표체,전반식유갑산(ATRA)유도린상세포암세포분화,RT-PCR검측분화전후정합소β1변화수평.결과 재고분화린상세포암중,정합소β1재종류단괴외주기저양세포정련속성표체,내측지단괴중앙가견산재혹도서상양성세포;재저분화린상세포암조직중,도서상양성세포명현증다,정미만분포;재A431세포중,정합소β1산재표체우종류세포.ATRA유도24h화48h후,ATRA처리조정합소β1여내삼조β-actin적RT-PCR산물전영광밀도치지비분별위0.071±0.025화0.029±0.018,명현저우대조조적0.148±0.027화0.136±0.011(P<0.05).결론 정합소β1재피부린상세포암조직배양종류세포중구유이질성표체모식,병수종류세포분화정도증고이표체하강,제시정합소β1여피부린상세포암발생급가능존재적종류간세포구유밀절관계.
Objective To study the expression of integrin β1 in squamous cell carcinoma(SCC)and explore the relationship between stem cell marker and SCC.Methods The expressions of integrin β1 in SCC tissues and SCC cell strain A431 were detected with immunohistochemical methods and cell staining method.The differentiation of SCC cells were induced with all-trans-retinoic acid(ATRA).The changes of integrin β1 levels before and after induction were detected with RT-PCR.Results In highly differentiated SCC tissues.integrin β1 was constantly expressed in the basal-like cells in the edge of tumor;some cells inside arranged as island also showed positive integrin β1 expression.In poorly differentiated SCC tissues.island-like integrin β1-positive cells remarkably increased and distributed in a diffuse way.In SCC A431 cells,integrin β1 was expressed unevenly in tumor cells.After treatment by ATRA,level of integrinβ1 mRNA in A431 cells significantly decreased compared with untreated control(P<0.05),and the ratios between the intensity values of intesrin β1 to β-actin were 0.071±0.025 and 0.029±0.018 at 24h and 48h,respectively,whereas in controls were 0.148 ±0.027 and0.136 ±0.011(P<0.05).Conclusions Integrin β1 is heterogeneously expressed in both SCC tissues and SCC A431 cells.The expression of Integrin β1 decreases when the differentiation level of tumor cells increase,indicating that integrin β1 is closely related with the initiation of SCC and potential cancer stem cells in SCC.