CAJ | 학술논문

目的观察高强度脉冲噪声暴露后豚鼠听功能及耳蜗结构的变化,探讨用于毛细胞再生研究的噪声性聋动物模型的建立方法 .方法健康成年白色红H豚鼠50只,雌雄不限,体重250～300g.随机分成2组,正常对照组10只,噪声暴露组40只.给予脉冲噪声(压力峰值为175.0 dB SPL,脉宽0.25 ms,间隔时间20秒)连续暴露200次.于噪声暴露前及暴露后1周、4周、8周检测听性脑干反应(auditory brainstem respons,ABR),毛细胞计数及耳蜗铺片免疫组化观察耳蜗结构变化.结果高强度脉冲噪声暴露后1周,40只豚鼠中有21只(52.5%)双耳各频率ABR阈值≥95 dB SPL.继续观察至噪声暴露后4周及8周,ABR阈值没有恢复,1周、4周、8周各频率ABR阈值比较无统计学差异(P>0.05).毛细胞计数结果显示,噪声暴露致极重度聋后1周,内毛细胞平均缺失率为91.4%,外毛细胞平均缺失率为97.2%.免疫组化染色分析结果显示,噪声暴露致聋后1周,内、外毛细胞胞核大部分缺失,内毛细胞内侧及外毛细胞外侧的支持细胞的胞核存在.结论高强度脉冲噪声暴露可造成豚鼠极重度感音神经性聋,耳蜗毛细胞广泛缺失且无法自行恢复,而支持细胞大部分存留,是进行毛细胞再生研究的理想动物模型.
목적 관찰고강도맥충조성폭로후돈서은공능급이와결구적변화,탐토용우모세포재생연구적조성성롱동물모형적건립방법 .방법 건강성년백색홍H돈서50지,자웅불한,체중250～300g.수궤분성2조,정상대조조10지,조성폭로조40지.급여맥충조성(압력봉치위175.0 dB SPL,맥관0.25 ms,간격시간20초)련속폭로200차.우조성폭로전급폭로후1주、4주、8주검측은성뇌간반응(auditory brainstem respons,ABR),모세포계수급이와포편면역조화관찰이와결구변화.결과 고강도맥충조성폭로후1주,40지돈서중유21지(52.5%)쌍이각빈솔ABR역치≥95 dB SPL.계속관찰지조성폭로후4주급8주,ABR역치몰유회복,1주、4주、8주각빈솔ABR역치비교무통계학차이(P>0.05).모세포계수결과현시,조성폭로치겁중도롱후1주,내모세포평균결실솔위91.4%,외모세포평균결실솔위97.2%.면역조화염색분석결과 현시,조성폭로치롱후1주,내、외모세포포핵대부분결실,내모세포내측급외모세포외측적지지세포적포핵존재.결론 고강도맥충조성폭로가조성돈서겁중도감음신경성롱,이와모세포엄범결실차무법자행회복,이지지세포대부분존류,시진행모세포재생연구적이상동물모형.
Objective To study the change of the guinea pig cochlea after high-intensity impulse noise exposure in order to assess its effects on hearing,and to discuss the methods of the noise deafness animal model for cochlea hair cell regeneration research.Methods Fifty adult guinea pigs were used,weight between 250 and 300g,and were divided into two groups in random.Fourty animals were incessant exposed to impulse noise 200 times (Pressure peak was 175.0 dB SPL,and Pulse duration was 0.25 ms,interval time 20s).For exposed group,10 were used as control group.Cochlear hair cell counting,Immunohistochemical-dyeing cochlea stretched preparation,and Audtory brainstem response (ABR) thresholds were determined before and 1 week,4 weeks and 8 weeks after exposed.Results One week after exposed to high-intensity impulse noise,there were 21 of the 40 guinea pigs (52.5%),with ABR thresholds≥95 dB SPL at every frequency,but not recovered after 8 weeks.No significant change of the ABR thresholds between 1 week,4 weeks and 8 weeks (P>0.05).Cochlea hair cell counting showed the average miss rate of inner hair cell is 91.4%;the outer hair cell is 97.2% in lweek after noise exposure, Immunohistochemical results showed,that most of the nucleus of the inner hair cell and outer hair cell were missed,but the supporting cell nucleus of the inner hair cell medial and outer hair cell lateral still existed in one week after noise exposure.Conclusion High-intensity impulse noise exposure may cause severe sensorineural deafness,and cochlear hair cells were generally missed and can not recover automatically,but most of the supporting cells were existed.This noise deafness animal model is promise of an ideal animal model for the further study for acoustic hair cell regeneration.