中华医学超声杂志（电子版）
中華醫學超聲雜誌（電子版）
중화의학초성잡지（전자판）
CHINESE JOURNAL OF MEDICAL ULTRASOUND(ELECTRONICAL VISION)
2010年
12期
2025-2030
,共6页
超声检查%前列腺肿瘤%细胞膜通透性
超聲檢查%前列腺腫瘤%細胞膜通透性
초성검사%전렬선종류%세포막통투성
Ultrasonography%Prostatic neoplams%Cell membrane permeability
目的 探讨低强度超声照射下前列腺癌细胞的细胞膜通透性变化.方法 频率为1 MHz低强度超声以连续模式照射6孔板中的单层贴壁LNCaP前列腺癌细胞,以正常细胞膜非通透性荧光染料-钙黄绿素(Calcein)标记细胞膜通透性增加的细胞,以核酸荧光染料-碘化丙啶(PI)标记死亡细胞.在声强为160 mW/cm2超声下照射5 s,荧光显微镜观察细胞膜通透性改变,扫描电镜观察细胞膜的形态变化;分别在不同低声强(80 mW/cm2、120 mW/cm2和160 mW/cm2)超声下照射5 s,另外在声强为120 mW/cm2超声下分别照射5 s、10 s和15 s,流式细胞仪分析细胞膜通透性增加的细胞比例与声强及照射时间的对应关系.结果 低强度超声作用后,荧光显微镜显示部分单层贴壁LNCaP前列腺癌细胞摄取Calcein而呈现绿色荧光,而对照组无细胞摄取Calcein;扫描电镜显示正常LNCaP前列腺癌细胞表面有密集微绒毛,而超声作用后细胞膜表面绒毛稀少、变平,少数细胞表面出现裂孔;流式细胞仪显示细胞膜通透性增加的细胞比例分别随声强和照射时间的增加而增加.结论 低强度超声可以增加前列腺癌细胞膜通透性,细胞膜通透性增加的细胞表面变平,少数出现裂孔;细胞膜通透性增加的细胞比例与声强和照射时间均呈正相关.
目的 探討低彊度超聲照射下前列腺癌細胞的細胞膜通透性變化.方法 頻率為1 MHz低彊度超聲以連續模式照射6孔闆中的單層貼壁LNCaP前列腺癌細胞,以正常細胞膜非通透性熒光染料-鈣黃綠素(Calcein)標記細胞膜通透性增加的細胞,以覈痠熒光染料-碘化丙啶(PI)標記死亡細胞.在聲彊為160 mW/cm2超聲下照射5 s,熒光顯微鏡觀察細胞膜通透性改變,掃描電鏡觀察細胞膜的形態變化;分彆在不同低聲彊(80 mW/cm2、120 mW/cm2和160 mW/cm2)超聲下照射5 s,另外在聲彊為120 mW/cm2超聲下分彆照射5 s、10 s和15 s,流式細胞儀分析細胞膜通透性增加的細胞比例與聲彊及照射時間的對應關繫.結果 低彊度超聲作用後,熒光顯微鏡顯示部分單層貼壁LNCaP前列腺癌細胞攝取Calcein而呈現綠色熒光,而對照組無細胞攝取Calcein;掃描電鏡顯示正常LNCaP前列腺癌細胞錶麵有密集微絨毛,而超聲作用後細胞膜錶麵絨毛稀少、變平,少數細胞錶麵齣現裂孔;流式細胞儀顯示細胞膜通透性增加的細胞比例分彆隨聲彊和照射時間的增加而增加.結論 低彊度超聲可以增加前列腺癌細胞膜通透性,細胞膜通透性增加的細胞錶麵變平,少數齣現裂孔;細胞膜通透性增加的細胞比例與聲彊和照射時間均呈正相關.
목적 탐토저강도초성조사하전렬선암세포적세포막통투성변화.방법 빈솔위1 MHz저강도초성이련속모식조사6공판중적단층첩벽LNCaP전렬선암세포,이정상세포막비통투성형광염료-개황록소(Calcein)표기세포막통투성증가적세포,이핵산형광염료-전화병정(PI)표기사망세포.재성강위160 mW/cm2초성하조사5 s,형광현미경관찰세포막통투성개변,소묘전경관찰세포막적형태변화;분별재불동저성강(80 mW/cm2、120 mW/cm2화160 mW/cm2)초성하조사5 s,령외재성강위120 mW/cm2초성하분별조사5 s、10 s화15 s,류식세포의분석세포막통투성증가적세포비례여성강급조사시간적대응관계.결과 저강도초성작용후,형광현미경현시부분단층첩벽LNCaP전렬선암세포섭취Calcein이정현록색형광,이대조조무세포섭취Calcein;소묘전경현시정상LNCaP전렬선암세포표면유밀집미융모,이초성작용후세포막표면융모희소、변평,소수세포표면출현렬공;류식세포의현시세포막통투성증가적세포비례분별수성강화조사시간적증가이증가.결론 저강도초성가이증가전렬선암세포막통투성,세포막통투성증가적세포표면변평,소수출현렬공;세포막통투성증가적세포비례여성강화조사시간균정정상관.
Objective To investigate the permeability changes of prostate cancer cells membrane under low intensity ultrasound in vitro.Methods The culture of monolayer adherent LNCaP prostate cancer cells in six-well plate was exposed to continuous ultrasound at frequency of 1 MHz.The cells membrane permeability (stained with Calcein)and cells viability(stained with PI)were evaluated by fluorescent microscope (FM) and cells morphological changes were evaluated by scanning electron microscope (SEM) under ultrasound with acoustic intensity of 160 mW/cm2 for 5 s.The rate of cells with increased cells membrane permeability as function of acoustic intensity (80 mW/cm2,120 mW/cm2 and 160 mW/cm2,for 5 s) and exposure duration (5 s,10 s and 15 s,acoustic intensity of 120 mW/cm2) was evaluated by flow cytometry.ResultsAfter low intensity of ultrasound,the cells with increased cell membrane permeability could be clearly shown with Calcein uptake under FM while no cell showed Calcein uptake in the control group.The SEM showed less microvilli on the cells after low intensity of ultrasound exposure and few cells showed holes on the cell membrane.The rate of cells with increased membrane permeability increased with acoustic intensity and exposure duration.Conclusion Low intensity ultrasound alone could increase membrane permeability of prostate cancer cells and cells with increased membrane permeability showed surface plane,uncommon holes on the cells membrane.The rate of cells with increased membrane permeability positively correlated with acoustic intensity and exposure duration.
