CAJ | 학술논문

以木薯华南205为试材,采用苯酚抽提法提取叶片蛋白质,并通过双向电泳技术研究影响木薯叶片蛋白电泳分析图谱的主要因素。结果表明：pH 4～7的IPG胶条可提高特定范围蛋白质点的分辨率;上样量200μg可得到较好的双向电泳图谱。本研究不仅为进一步研究木薯叶片蛋白电泳分析提供技术手段,同时也为分析木薯块根、茎和繁殖器官的蛋白质提供参考。
이목서화남205위시재,채용분분추제법제취협편단백질,병통과쌍향전영기술연구영향목서협편단백전영분석도보적주요인소。결과표명：pH 4～7적IPG효조가제고특정범위단백질점적분변솔;상양량200μg가득도교호적쌍향전영도보。본연구불부위진일보연구목서협편단백전영분석제공기술수단,동시야위분석목서괴근、경화번식기관적단백질제공삼고。
South China 205（SC105） was adopted in this experiment to compare different effects of two-dimensional electrophoresis（2-DE） gels.Phenol extraction method was used to extract proteins.The results showed that immobilized pH gradients（IPG） strip with pH 4~7 can improve protein resolution in certain range.Sample loading of 200 mg produced better 2-DE gel image.The results not only provides techniques for getting a good 2-DE profile of cassava leaf,also for tuberous root,stem and other reproduction organ.