CAJ | 학술논문

目的研究吸毒人群中艾滋病病毒(HIV)和丙型肝炎病毒(HCV)感染者与未感染者中,等位基因树突状细胞表面特异性分子同源物(DC-SIGNR)的分布情况,筛选出HIV或HCV抗性基因.方法选择深圳市戒毒所吸毒人群血液样本500份,其中313份来自静脉注射吸毒者,对他们进行HIV及HCV抗体筛查,并提取外周血基因组DNA,对DC-SIGNR颈区基因进行聚合酶链反应(PCR)扩增,对扩增产物进行琼脂糖凝胶电泳检测和基因序列测定分析.结果检出97例HIV阳性吸毒者,均为静脉注射吸毒者,且全部为HCV阳性;HIV阴性吸毒人群中,HCV抗体阳性率57.57%(232/403);HIV阴性静脉注射吸毒者中,HCV阳性率63.89%(138/216);而采用鼻吸等其他方式吸毒者的阳性率为50.26%(94/187),静脉注射吸毒与其他方式吸毒者阳性率的差异具有统计学意义(x2=7.61,P=0.0058).x2检验结合确切概率法分析显示,HIV阳性的吸毒者中,DC-SIGNR颈区5/6和5/8等位基因型的出现频率[4.55%(4/88)和2.27%(2/88)]高于HIV未感染组[1.04%(4/384)和0(0/384),确切概率法P=0.043和P=0.034],具有统计学意义;HCV阳性吸毒人群中,各类杂合子及纯合子的出现频率与HCV阴性吸毒人群间差异无统计学意义,静脉注射吸毒者与其他方式吸毒者之间差异无统计学意义.结论在吸毒人群中,DC-SIGNR颈区等位基因分布与HCV感染无明显相关性,而等位基因型5/6可能与HIV感染相关,但由于频率较低,仍需进一步验证.
목적 연구흡독인군중애자병병독(HIV)화병형간염병독(HCV)감염자여미감염자중,등위기인수돌상세포표면특이성분자동원물(DC-SIGNR)적분포정황,사선출HIV혹HCV항성기인.방법 선택심수시계독소흡독인군혈액양본500빈,기중313빈래자정맥주사흡독자,대타문진행HIV급HCV항체사사,병제취외주혈기인조DNA,대DC-SIGNR경구기인진행취합매련반응(PCR)확증,대확증산물진행경지당응효전영검측화기인서렬측정분석.결과 검출97례HIV양성흡독자,균위정맥주사흡독자,차전부위HCV양성;HIV음성흡독인군중,HCV항체양성솔57.57%(232/403);HIV음성정맥주사흡독자중,HCV양성솔63.89%(138/216);이채용비흡등기타방식흡독자적양성솔위50.26%(94/187),정맥주사흡독여기타방식흡독자양성솔적차이구유통계학의의(x2=7.61,P=0.0058).x2검험결합학절개솔법분석현시,HIV양성적흡독자중,DC-SIGNR경구5/6화5/8등위기인형적출현빈솔[4.55%(4/88)화2.27%(2/88)]고우HIV미감염조[1.04%(4/384)화0(0/384),학절개솔법P=0.043화P=0.034],구유통계학의의;HCV양성흡독인군중,각류잡합자급순합자적출현빈솔여HCV음성흡독인군간차이무통계학의의,정맥주사흡독자여기타방식흡독자지간차이무통계학의의.결론 재흡독인군중,DC-SIGNR경구등위기인분포여HCV감염무명현상관성,이등위기인형5/6가능여HIV감염상관,단유우빈솔교저,잉수진일보험증.
Objective To study the distribution of DC-SIGN/DC-SIGNR alleles among drug user (DUs)populations with or without HIV/HCV infection in Shenzhen,and to evaluate the role of these alleles in the construction of genetic resistance to HIV or HCV and screen out the anti-HIV/HCV gene in Shenzhen.Methods All 500 DU blood samples were collected from Shenzhen Detoxification Center,including 313 from injected drug users(IDUs).All samples were screened for HIV and HCV antibody by means of ELISA.The genomic DNA were extracted and amplified bv PCR The neck domain repeat regions of DC-SIGN/DC-SIGNR were sequenced directly from the PCR products to confirm the amplification for some samples and all positive PCR products were analyzed by agarose gel electrophoresis.Results Of 500 samples,97 were found HIV positive,all of which were IDUs and HCV positive.The total positive rate of HCV among all HIV negative DU was 57.57%(232/403),and it was 63.89%(138/216)among IDUs;in comparing to the 50.26%(94/187)of DUs with other manners there showed significant difference(x2=7.61,P=0.0058).Among HIV+DUs,there was a higher proportion of patient with the DC-SIGNR 5/6 and 5/8(Fisher's exact,P=0.043 and P=0.034)with statistical significance:there was no statistically significant difierence between HCV+and HCV-DUs and no significant difierenee between IDUs and other DUs for the DC-SIGNR polymorphisrn.Conclusion The results might indicate that DC-SIGN/DC-SIGNR polymorphism might not influence the susceptibility to HCV.Genotype 5/6 might probably have a relation with HIV infection,but still need further investigation for the low frequency.