CAJ | 학술논문

目的:进一步研究半边旗的抗肿瘤活性成分.方法:运用反复硅胶柱层析的方法对半边旗中的化学成分进行分离纯化,以LC-MS及各种有机波谱法鉴定化学单体结构;并通过MTT法对分离鉴定的单体化合物及其苷元进行抗肿瘤活性研究.结果:从中分离鉴定1个二萜苷类化合物,为11β-hydroxy-15-oxo-ent-kaur-16-en-19-oic acid 19-β-D-glucoside(Ⅰ);化合物Ⅰ及其苷元5F均可抑制两种肿瘤细胞A549和CNE-2Z的生长,且它们对两种肿瘤细胞的抑制作用呈一定剂量依赖关系和时间依赖关系,化合物5F的活性强于化合物Ⅰ.结论:α,β不饱和环戊酮结构是其活性部位,5F中19位碳上的羧基与糖连接形成酯苷,成为化合物Ⅰ后,其抗肿瘤活性大大降低.
목적:진일보연구반변기적항종류활성성분.방법:운용반복규효주층석적방법대반변기중적화학성분진행분리순화,이LC-MS급각충유궤파보법감정화학단체결구;병통과MTT법대분리감정적단체화합물급기감원진행항종류활성연구.결과:종중분리감정1개이첩감류화합물,위11β-hydroxy-15-oxo-ent-kaur-16-en-19-oic acid 19-β-D-glucoside(Ⅰ);화합물Ⅰ급기감원5F균가억제량충종류세포A549화CNE-2Z적생장,차타문대량충종류세포적억제작용정일정제량의뢰관계화시간의뢰관계,화합물5F적활성강우화합물Ⅰ.결론:α,β불포화배무동결구시기활성부위,5F중19위탄상적최기여당련접형성지감,성위화합물Ⅰ후,기항종류활성대대강저.
AIM: To further study the antitumor constituents of Pteris semipinnata L. METHODS: Compounds were isolated by consecutive silica gel column chromatography. Their structures were elucidated by spectroscopic methods. Tumor cytotoxicity of the compounds was screened by using standard MTT method. RESULTS: One compound was isolated from Pteris semipinnata L. , and its structure was identified as 11β-hydroxy-15-oxo-ent-kaur-16-en-19-oic acid 19-β-D-glucoside(Ⅰ). Compound I and its aglycone 5F showed to have inhibitory action a-gainst two cancer cell lines A549 and CNE-2Z and showed the dose-response relation. But the antitumor activity of compound 5F was stronger than that of compound Ⅰ. CONCLUSION: The antitumor active site in the stueture is α,β-methylene cyclopentanone moiety. Nineteen position carboxylic group in 5F has to be uncombined with sugar residue to form ester linkage (compound Ⅰ ) otherwise antitumour activity is lost.