CAJ | 학술논문

目的调查北京地区肠道门诊就诊腹泻患者人杯状病毒的感染情况.方法收集北京市2011年4月至2012年3月丰台、昌平、怀柔3个区县的肠道门诊就诊腹泻患者450例,采集患者粪便标本,使用逆转录聚合酶链反应法(RT-PCR)对粪便标本进行人杯状病毒RNA检测,对RT-PCR阳性标本的PCR产物进行克隆测序.结果 450例患者标本中68例人杯状病毒阳性(68/450,15.11％).选择其中18例PCR产物进行克隆测序,将获得的序列进行比对分析、构建系统发生树,结果表明,15株为诺如病毒,3株为扎如病毒.其中诺如病毒GⅡ/4型11株(11/18,61.11％),GⅡ/7型1株(1/18,5.56％),GⅡ组未定型3株(3/18,16.67％).结论人杯状病毒是北京地区肠道门诊就诊腹泻患者的重要病原,主要流行株为诺如病毒GⅡ/4型.
목적 조사북경지구장도문진취진복사환자인배상병독적감염정황.방법 수집북경시2011년4월지2012년3월봉태、창평、부유3개구현적장도문진취진복사환자450례,채집환자분편표본,사용역전록취합매련반응법(RT-PCR)대분편표본진행인배상병독RNA검측,대RT-PCR양성표본적PCR산물진행극륭측서.결과 450례환자표본중68례인배상병독양성(68/450,15.11％).선택기중18례PCR산물진행극륭측서,장획득적서렬진행비대분석、구건계통발생수,결과표명,15주위낙여병독,3주위찰여병독.기중낙여병독GⅡ/4형11주(11/18,61.11％),GⅡ/7형1주(1/18,5.56％),GⅡ조미정형3주(3/18,16.67％).결론 인배상병독시북경지구장도문진취진복사환자적중요병원,주요류행주위낙여병독GⅡ/4형.
Objective To investigate the characteristics of human calicivirus infections among diarrhea patients visiting enteric clinics in Beijing.Methods From April 2011 to March 2012,450 diarrhea patients visiting enteric clinics were investigated in Beijing,and fecal specimens of the patients were collected.Human caliciviruses were detected by a reverse transcription polymerase chain reaction (RT-PCR),and then the PCR products were cloned and sequenced.Results A total of 68 positive cases were detected among 450 patients with diarrhea,with the positive rate of 15.11％.Eighteen PCR products were randomly selected for sequencing.Multi-sequence aligements were performed,and phylogenetic tree was constructed.Results showed that 3 strains were sapoviruses and 15 strains were noroviruses,among which 11 strains belonged to norovirus GⅡ/4 (11/18,61.11％),1 strains belonged to norovirus GⅡ/7 (1/18,5.56％),the other 3 strains were not typed (3/18,16.67％) Conclusions Human calicivirus was the important pathogen of diarrhea patients visiting enteric clinics in Beijing,and norovirus GⅡ/4 was the predominant genotype.