国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2010年
9期
603-606,封3
,共5页
重症急性胰腺炎%CO2气腹%白细胞介素-6%E-选择素%P-选择素
重癥急性胰腺炎%CO2氣腹%白細胞介素-6%E-選擇素%P-選擇素
중증급성이선염%CO2기복%백세포개소-6%E-선택소%P-선택소
Severe acute pancreatitis%CO2 pneumoperitoneum%Interleukin-6%E-selectin%P-selectin
目的 研究腹腔镜手术时CO2气腹对SD大鼠重症急性胰腺炎病变局部、血淀粉酶、白细胞介素-6(IL-6)、E-选择素和P-选择素的影响. 方法 雄性SD大鼠50只,随机分为3组:CO2气腹组(n=20),5%牛磺胆酸钠胆胰管逆行注射方法制备SAP动物模型后,以气腹机向大鼠腹腔内注入CO2(压力12 mm Hg,维持30 min);SAP组(n=20):建立SAP模型后关腹,不充入CO2;单纯手术组(n=10):仅开腹翻动胰腺后关腹.各组均于术后2.5 h处死动物,取静脉血测定血淀粉酶、白细胞介素-6、E-选择素和P-选择素的水平,并进行胰腺组织病理学检查. 结果 与单纯手术组相比,CO2气腹组和SAP组病理学评分、血清淀粉酶、白细胞介素-6、E-选择素和P-选择索均明显升高,差异有统计学意义(P=0.000).与SAP组相比,CO2气腹组血白细胞介素-6明显降低,差异有统计学意义P=0.000;病理学评分(P=0.294)、血清淀粉酶(P=0.073)、E-选择素(P=0.126)和P-选择素(P=0.213)的差异无统计学意义. 结论 对于SAP大鼠,CO2气腹对白细胞介素-6有一定的抑制作用;对胰腺病理变化及E-选择素和P-选择素无明显影响.
目的 研究腹腔鏡手術時CO2氣腹對SD大鼠重癥急性胰腺炎病變跼部、血澱粉酶、白細胞介素-6(IL-6)、E-選擇素和P-選擇素的影響. 方法 雄性SD大鼠50隻,隨機分為3組:CO2氣腹組(n=20),5%牛磺膽痠鈉膽胰管逆行註射方法製備SAP動物模型後,以氣腹機嚮大鼠腹腔內註入CO2(壓力12 mm Hg,維持30 min);SAP組(n=20):建立SAP模型後關腹,不充入CO2;單純手術組(n=10):僅開腹翻動胰腺後關腹.各組均于術後2.5 h處死動物,取靜脈血測定血澱粉酶、白細胞介素-6、E-選擇素和P-選擇素的水平,併進行胰腺組織病理學檢查. 結果 與單純手術組相比,CO2氣腹組和SAP組病理學評分、血清澱粉酶、白細胞介素-6、E-選擇素和P-選擇索均明顯升高,差異有統計學意義(P=0.000).與SAP組相比,CO2氣腹組血白細胞介素-6明顯降低,差異有統計學意義P=0.000;病理學評分(P=0.294)、血清澱粉酶(P=0.073)、E-選擇素(P=0.126)和P-選擇素(P=0.213)的差異無統計學意義. 結論 對于SAP大鼠,CO2氣腹對白細胞介素-6有一定的抑製作用;對胰腺病理變化及E-選擇素和P-選擇素無明顯影響.
목적 연구복강경수술시CO2기복대SD대서중증급성이선염병변국부、혈정분매、백세포개소-6(IL-6)、E-선택소화P-선택소적영향. 방법 웅성SD대서50지,수궤분위3조:CO2기복조(n=20),5%우광담산납담이관역행주사방법제비SAP동물모형후,이기복궤향대서복강내주입CO2(압력12 mm Hg,유지30 min);SAP조(n=20):건립SAP모형후관복,불충입CO2;단순수술조(n=10):부개복번동이선후관복.각조균우술후2.5 h처사동물,취정맥혈측정혈정분매、백세포개소-6、E-선택소화P-선택소적수평,병진행이선조직병이학검사. 결과 여단순수술조상비,CO2기복조화SAP조병이학평분、혈청정분매、백세포개소-6、E-선택소화P-선택색균명현승고,차이유통계학의의(P=0.000).여SAP조상비,CO2기복조혈백세포개소-6명현강저,차이유통계학의의P=0.000;병이학평분(P=0.294)、혈청정분매(P=0.073)、E-선택소(P=0.126)화P-선택소(P=0.213)적차이무통계학의의. 결론 대우SAP대서,CO2기복대백세포개소-6유일정적억제작용;대이선병리변화급E-선택소화P-선택소무명현영향.
Objective To examine the effect of carbon dioxide pneumoperitoneum on local pancreas pathological changes, serum levels of amylase, IL-6, E-selectin and P-selectin in rats with severe acute pancreatitis (SAP). Methods Fifty healthy male SD rats were randomly divided into 3 groups: CO2 experiment group ( n =20): SAP was induced by injecting 5% sodium taurocholate through retrogradely common biliopancreatic ducts via duodenal papilla,and then CO2 pneumoperitoneum was established at a pressure of 12 mmHg for 30 min; SAP group ( n = 20): The rats were treated as same as CO2 experiment group, excepting CO2 pneumoperitoneum; Control group (n = 10): Abdomen was incised and nothing was done to duodenum and pancreas except for moving them softly. The blood samples were gathered for examining serum levels of amylase, IL-6, E-selectin and P-selectin, and histopathologic examination of pancreas was done.Results Compared with control group, the pancreatic pathologic histology score, serum levels of amylase,IL-6, E-selectin and P-selectin were significantly higher in the CO2 experiment group and SAP group (P =0.000). The levels of IL-6 were significantly lower in CO2 experiment group compared to SAP group (P =0. 000). There was no significant difference between CO2 experiment group and SAP group in pancreatic pathologic histology score( P =- 0.294), the levels of serum amylase ( P = 0.073 ), E-selectin and P-selectin. Conclusions CO2 pneumoperitoneum has no adverse effect on the changes of pathological histopathology and the levels of IL-6, E-selectin and P-selectin in SD rats with SAP.
