CAJ | 학술논문

目的研究中华眼镜蛇毒膜毒素(MT-12)对人膀胱癌细胞株EJ-m3的作用,并探讨其作用机制.方法以穿透人工基底膜能力为依据筛选具有体外高侵袭能力的膀胱癌细胞;采用四氮唑蓝(MTY)法检测MT-12不同浓度对EJ-m3细胞的生长抑制率;同时流式细胞仪检测不同浓度MT-12作用24h后细胞中趋化因子受体CXCR4的表达.结果经过反复筛选获得膀胱癌细胞EJ的体外高侵袭力亚系,EJ-m3.MT-12可有效抑制FJ-m3细胞的生长,具有浓度依赖性特点.MT-12对EJ-m3作用72h的ICSO是0.66ug/mL;流式细胞仪检测结果显示,在0.125～0.5ug/mL,MT-12组随着药物浓度的增加Cx-CR4的表达逐渐减弱(P=0.020).结论 MT-12可有效抑制膀胱癌细胞生长,其作用机制可能与CXCR4在膀胱癌细胞中高表达有关.MT-12有潜力成为新的抗膀胱癌药物.
목적 연구중화안경사독막독소(MT-12)대인방광암세포주EJ-m3적작용,병탐토기작용궤제.방법 이천투인공기저막능력위의거사선구유체외고침습능력적방광암세포;채용사담서람(MTY)법검측MT-12불동농도대EJ-m3세포적생장억제솔;동시류식세포의검측불동농도MT-12작용24h후세포중추화인자수체CXCR4적표체.결과 경과반복사선획득방광암세포EJ적체외고침습력아계,EJ-m3.MT-12가유효억제FJ-m3세포적생장,구유농도의뢰성특점.MT-12대EJ-m3작용72h적ICSO시0.66ug/mL;류식세포의검측결과 현시,재0.125～0.5ug/mL,MT-12조수착약물농도적증가Cx-CR4적표체축점감약(P=0.020).결론 MT-12가유효억제방광암세포생장,기작용궤제가능여CXCR4재방광암세포중고표체유관.MT-12유잠력성위신적항방광암약물.
Objectives To investigate the effects of the membrane toxin 12 (MT-12) from Naja naja atra venom on human bladder cancer cell EJ-m3 and study its mechanisms. Methods Using a specially constructed in vitro invasion chamber cells that penetrate the Matrigel were selected and cultured to establish offspring cell lines;The growth inhibition rates of EJ-m3 cell were investigated by MTT method with various concentration MT-12.chemokine receptor CXCR4 expressions were detected by flow cytometry after 24 hours with various concentration MT -12. Results Acquire offspring cell lines nime EJ-m3 cell from bladder cancer cell EJ by selected again and a-gain. MT-12 effectively inhibited the growth of EJ-m3 cell,which depended on concentration of the drug. The IC50 after 72 hours with MT-12 on EJ-m3 ceil were 0.66ug/mL, flow cytometry results indicated that the expres-sion of CXCR4 gradually decreased with the increase of MT-12 dose(0. 125-0.5ug/mL group, p =0. 020).Conclusions MT-12 could significantly inhibit the growth of bladder cancer cells, The strong expression of CXCR4 might be one of its action mechanisms. MT-12 are largely potential anticancer drug for bladder carcinoma.