CAJ | 학술논문

目的探讨牛磺酸对反复力竭运动大鼠骨骼肌线粒体的保护作用.方法雄性SD大鼠30只随机分为安静对照组、力竭对照组、牛磺酸组,每组10只.安静对照组和力竭对照组大鼠每天按0.8 mL/kg灌喂生理盐水1次;牛磺酸组大鼠每天用200 mg/L牛磺酸按200 mg/kg灌喂1次.力竭对照组和牛磺酸组大鼠运动前先连续灌喂15d,力竭运动期间(30 d)每天继续灌喂.安静对照组大鼠整个实验过程中不运动,只灌喂生理盐水,灌喂时间与力竭对照组和牛磺酸组一样,共45 d.运动后,处死大鼠,切取股四头肌,并对大鼠体质量及股四头肌湿重进行比较;行透射电镜观察,着重观察股四头肌线粒体形态.结果力竭对照组体质量、股四头肌湿重显著低于安静对照组和牛磺酸组,力竭对照组大鼠骨骼肌肌丝排列紊乱,线粒体肿胀和空泡变性,牛磺酸组大部分骨骼肌超微结构图接近正常.结论口服牛磺酸对反复力竭运动大鼠骨骼肌线粒体具有保护作用,它减少反复力竭收缩骨骼肌的坏死丢失.
목적 탐토우광산대반복력갈운동대서골격기선립체적보호작용.방법 웅성SD대서30지수궤분위안정대조조、력갈대조조、우광산조,매조10지.안정대조조화력갈대조조대서매천안0.8 mL/kg관위생리염수1차;우광산조대서매천용200 mg/L우광산안200 mg/kg관위1차.력갈대조조화우광산조대서운동전선련속관위15d,력갈운동기간(30 d)매천계속관위.안정대조조대서정개실험과정중불운동,지관위생리염수,관위시간여력갈대조조화우광산조일양,공45 d.운동후,처사대서,절취고사두기,병대대서체질량급고사두기습중진행비교;행투사전경관찰,착중관찰고사두기선립체형태.결과 력갈대조조체질량、고사두기습중현저저우안정대조조화우광산조,력갈대조조대서골격기기사배렬문란,선립체종창화공포변성,우광산조대부분골격기초미결구도접근정상.결론 구복우광산대반복력갈운동대서골격기선립체구유보호작용,타감소반복력갈수축골격기적배사주실.
Objective To investigate the protective effect of taurine on the skeletal muscle mitochondria in rats subjected to repeated exhausting exercises on a treadmill. Methods Thirty SD male rats were randomized into a quiet control (QC) group ( n =10),an exhausting exercise control (EEC) group ( n =10) and an exhausting exercise plus taurine treatment (TT) group ( n =10).QC and EEC groups were fed with normal saline (dose:0.8 mL/kg) once per day;,TT group was fed with taurine (concentration:200 mg/L; dose:200 mg/kg) once per day.The EEC and TT groups were fed continuously for 15 days pre-exercise and 30 days during the exhausting exercises.The rats in the QC group were subjected to no exercise other than the feeding for 45 days as in the other 2 groups.After repeated exhausting running on a treadmill in a course of 30 days,all the rats were sacrificed to harvest the quadriceps femoris muscles.The body mass of the rats and the net mass of the quadriceps femoris were measured.Transmission electron microscopy was used to observe the morphology of the mitochondria. Results The body mass of the rats and the net mass of the quadriceps femoris were obviously lower in the EEC group than in the other 2 groups.The myofilaments in the quadriceps femoris were deranged,the mitochondria swelling and the vacuole degenerated in the EEC group.The ultra-structures of the quadriceps femoris and mitochondria were roughly normal in the TT group as in the QC group. Conclusion Oral taurine administration can protect and improve the mitochondrial function in the skeletal muscles in rats taking repeated exhausting exercises,reducing necrosis and loss of the skeletal muscles caused by the exhausting exercises.