中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2008年
5期
542-545
,共4页
李仁哲%孙勤%杨刚毅%李伶%李钶%齐晓娅%朱伟%孙逊%唐毅%刘桦%Guenther Boden
李仁哲%孫勤%楊剛毅%李伶%李鈳%齊曉婭%硃偉%孫遜%唐毅%劉樺%Guenther Boden
리인철%손근%양강의%리령%리아%제효아%주위%손손%당의%류화%Guenther Boden
内脂素%胰岛素钳夹%胰岛素敏感性%成纤维细胞生长因子21
內脂素%胰島素鉗夾%胰島素敏感性%成纖維細胞生長因子21
내지소%이도소겸협%이도소민감성%성섬유세포생장인자21
Visfatin%Euglycemic-hyperinsulinemia clamp%Insulin sensitivity%Fibroblast growth factor-21
目的 探讨内脂素基因过表达对大鼠胰岛素敏感性和血浆成纤维细胞生长因子21(FGF-21)的影响.方法 构建大鼠内脂素基因重组真核表达质粒,将该质粒转染大鼠.在转染前后采用正糖-高胰岛素钳夹技术评价大鼠胰岛素敏感性变化,并采用放射免疫法测定其血浆FGF-21浓度.结果 在质粒注射后3 d大鼠血清内脂素水平明显升高(1.49±0.06 vs 0.99±0.04,P<0.01);葡萄糖输注率(GIR)[(35.3±1.4 vs 27.6±1.7)mg·kg-1·min-1,P<0.01]较注射前明显升高;基础血浆胰岛素水平明显下降[(14.5±3.7 vs 24.4±6.2)mU/L,P<0.05];总胆固醇、高密度脂蛋白胆固醇明显降低[(1.31±0.10 vs1.76±0.22)mmol/L和(0.59±0.04 vs 0.95±0.15)mmol/L,均P<0.05];血浆FGF-21也降低[(2.25±0.19 vs 2.59±0.23) μg/L,P<0.05];在转染前后基础血糖和血浆脂联素水平无明显变化.结论 内脂素质粒转染使大鼠血浆vsfatin水平升高和FGF-21水平降低,胰岛素敏感性增强.
目的 探討內脂素基因過錶達對大鼠胰島素敏感性和血漿成纖維細胞生長因子21(FGF-21)的影響.方法 構建大鼠內脂素基因重組真覈錶達質粒,將該質粒轉染大鼠.在轉染前後採用正糖-高胰島素鉗夾技術評價大鼠胰島素敏感性變化,併採用放射免疫法測定其血漿FGF-21濃度.結果 在質粒註射後3 d大鼠血清內脂素水平明顯升高(1.49±0.06 vs 0.99±0.04,P<0.01);葡萄糖輸註率(GIR)[(35.3±1.4 vs 27.6±1.7)mg·kg-1·min-1,P<0.01]較註射前明顯升高;基礎血漿胰島素水平明顯下降[(14.5±3.7 vs 24.4±6.2)mU/L,P<0.05];總膽固醇、高密度脂蛋白膽固醇明顯降低[(1.31±0.10 vs1.76±0.22)mmol/L和(0.59±0.04 vs 0.95±0.15)mmol/L,均P<0.05];血漿FGF-21也降低[(2.25±0.19 vs 2.59±0.23) μg/L,P<0.05];在轉染前後基礎血糖和血漿脂聯素水平無明顯變化.結論 內脂素質粒轉染使大鼠血漿vsfatin水平升高和FGF-21水平降低,胰島素敏感性增彊.
목적 탐토내지소기인과표체대대서이도소민감성화혈장성섬유세포생장인자21(FGF-21)적영향.방법 구건대서내지소기인중조진핵표체질립,장해질립전염대서.재전염전후채용정당-고이도소겸협기술평개대서이도소민감성변화,병채용방사면역법측정기혈장FGF-21농도.결과 재질립주사후3 d대서혈청내지소수평명현승고(1.49±0.06 vs 0.99±0.04,P<0.01);포도당수주솔(GIR)[(35.3±1.4 vs 27.6±1.7)mg·kg-1·min-1,P<0.01]교주사전명현승고;기출혈장이도소수평명현하강[(14.5±3.7 vs 24.4±6.2)mU/L,P<0.05];총담고순、고밀도지단백담고순명현강저[(1.31±0.10 vs1.76±0.22)mmol/L화(0.59±0.04 vs 0.95±0.15)mmol/L,균P<0.05];혈장FGF-21야강저[(2.25±0.19 vs 2.59±0.23) μg/L,P<0.05];재전염전후기출혈당화혈장지련소수평무명현변화.결론 내지소질립전염사대서혈장vsfatin수평승고화FGF-21수평강저,이도소민감성증강.
Objective To investigate the effects of visfatin gene overexpressian on insulin sensitivity and plasma fibroblast growth factor 21 (FGF-21) level in rats. Methods The recombinant visfatin plasmid was constructed and transfected into normal rats. The euglyeemic-hyperinsulinemie clamp experiments were performed to evaluate the change of insulin sensitivity before and after administration of the plasmid, and plasma FGF-21 level was determined by radioimmunoassay. Results Plasma visfatin levels and glucose infusion rates (GIR) were significantly increased [(1.49±0.06 vs 0.99±0.04) and (35.3±1.4 vs 27.6±1.7) mg·kg-1·min-1,respectively, all P<0.01]. Fasting insulin level was significantly decreased [(14.5±3.7 vs 24.4±6.2) mU/L,P<0.05], total cholesterol and high-density lipoprotein cholesterol were significantly decreased [(1.31±0.10 vs1.76±0.22) mmol/L and (0.59±0.04 vs 0.95±0.15) mmol/L, respectively, all P<0.05], and plasma FGF-21 level was also decreased [(2.25±0.19 vs 2.59±0.23)μg/L, P<0.05] after plasmid being injected 3 days compared with that before injection, but there were no significant differences in fasting glucose and adiponectin levels before and after administration. Conclusion The transfection of visfatin plasmid increases plasma visfatin level, decreases FGF-21 levels and improves insulin sensitivity in normal rats.
