中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2012年
5期
517-520
,共4页
张秀春%张立霞%李伟红%王誓闻%孙玉兰%王圆圆%关增智%刘秀军%杨育松%张曙光%禹惠兰%张丽娟
張秀春%張立霞%李偉紅%王誓聞%孫玉蘭%王圓圓%關增智%劉秀軍%楊育鬆%張曙光%禹惠蘭%張麗娟
장수춘%장립하%리위홍%왕서문%손옥란%왕원원%관증지%류수군%양육송%장서광%우혜란%장려연
查菲埃立克体%嗜吞噬无形体%血清流行病学%间接免疫荧光方法
查菲埃立剋體%嗜吞噬無形體%血清流行病學%間接免疫熒光方法
사비애립극체%기탄서무형체%혈청류행병학%간접면역형광방법
Ehrlichia chaffeensis%Anaplasma phagocytophilum%Seroprevalence%Microindirect immunofluorescent assay
目的 调查北京市农村人群及家畜新发查菲埃立克体和嗜吞噬无形体感染状况.方法 按地理位置选择北京市西北(延庆)、东北(密云)及东南(通州)地区3个植被率较高的旅游县(区).按在册户籍随机以家庭为单位,调查并采集562人及90只山羊、71头牛和2只家犬血液标本,按WHO推荐间接免疫荧光方法(mIFA)检测血清IgG抗体.动物血球提取DNA扩增无形体16S rRNA基因并测序分析.结果 人群查菲埃立克体和嗜吞噬无形体抗体阳性率分别为16.5％和14.0％；山羊和牛嗜吞噬无形体抗体阳性率分别为2.3％和0；2只家犬未检测到相应抗体.山羊、牛无形体16S rRNA扩增阳性率分别为48.9％和23.9％.16S rRNA序列(228 bp)分析显示,3类优势流行株无明显地理分布特征.结论 北京市农村人群存在查菲埃立克体和嗜吞噬无形体感染,调查地区家畜存在无形体感染.
目的 調查北京市農村人群及傢畜新髮查菲埃立剋體和嗜吞噬無形體感染狀況.方法 按地理位置選擇北京市西北(延慶)、東北(密雲)及東南(通州)地區3箇植被率較高的旅遊縣(區).按在冊戶籍隨機以傢庭為單位,調查併採集562人及90隻山羊、71頭牛和2隻傢犬血液標本,按WHO推薦間接免疫熒光方法(mIFA)檢測血清IgG抗體.動物血毬提取DNA擴增無形體16S rRNA基因併測序分析.結果 人群查菲埃立剋體和嗜吞噬無形體抗體暘性率分彆為16.5％和14.0％；山羊和牛嗜吞噬無形體抗體暘性率分彆為2.3％和0；2隻傢犬未檢測到相應抗體.山羊、牛無形體16S rRNA擴增暘性率分彆為48.9％和23.9％.16S rRNA序列(228 bp)分析顯示,3類優勢流行株無明顯地理分佈特徵.結論 北京市農村人群存在查菲埃立剋體和嗜吞噬無形體感染,調查地區傢畜存在無形體感染.
목적 조사북경시농촌인군급가축신발사비애립극체화기탄서무형체감염상황.방법 안지리위치선택북경시서북(연경)、동북(밀운)급동남(통주)지구3개식피솔교고적여유현(구).안재책호적수궤이가정위단위,조사병채집562인급90지산양、71두우화2지가견혈액표본,안WHO추천간접면역형광방법(mIFA)검측혈청IgG항체.동물혈구제취DNA확증무형체16S rRNA기인병측서분석.결과 인군사비애립극체화기탄서무형체항체양성솔분별위16.5％화14.0％；산양화우기탄서무형체항체양성솔분별위2.3％화0；2지가견미검측도상응항체.산양、우무형체16S rRNA확증양성솔분별위48.9％화23.9％.16S rRNA서렬(228 bp)분석현시,3류우세류행주무명현지리분포특정.결론 북경시농촌인군존재사비애립극체화기탄서무형체감염,조사지구가축존재무형체감염.
Objective To investigate the status of Ehrlichia (E.)chaffeensis and A naplasma (A.) phagocytophilum infection among farming populations and domestic animals in the rural area of Beijing,China.Methods Blood samples from 562 farmers and 163 blood samples including 90 goats,71 ox and 2 dogs,were collected.Specificity of IgG antibodies against E.chaffeensis and A.phagocytophilum were tested by micro-indirect immunofluorescent assay (mIFA).16S rRNA genes of A.phagocytophilum were amplified from the domestic animal blood samples and products were sequenced and analyzed by nested PCR.Results The positive rates of E.chaffeensis and A.phagocytophilum antibody were 16.5％ and 14.0％ respectively for farmers.The total positive rates of A.phagocytophilum were 2.3％ and 0 for both goats and oxen respectively.No antibody was found for the 2 tested dogs.The PCR positive rates were 48.9％ and 23.9％ for goats and oxen respectively.Three dominant varieties of A.phagocytophilum were demonstrated in goats and oxen.Conclusion The prevalence rates of E.chaffeensis and A.phagocytophilum were identified in the rural areas of Beijing.
