CAJ | 학술논문

目的探讨关节软骨细胞-琼脂糖复合体三维培养模型内最利于各部分细胞生长的氧气和葡萄糖条件,为体外工程化软骨组织的构建提供合适的能量代谢环境. 方法根据极谱电极法原则,自行制作并应用针形氧电极测定1×107、2×107、4×107个/mL 3种不同细胞密度牛关节软骨细胞-琼脂糖体外三维复合体模型内立体空间1～5 mm各个深度位置的氧分压值,每种细胞密度模型6个,重复测量3次,分析立体空间氧分压的分布情况.在相同细胞密度模型中按是否加入葡萄糖分为无糖和低糖两组,测定培养液内葡萄糖浓度对模型内立体空间氧分压的影响. 结果在3种细胞密度的关节软骨细胞-琼脂糖复合体体外三维培养模型1～3 mm深度位置范围内,氧分压梯度变化最明显,氧分压值的差值高达50 mm Hg(1mm Hg=0.13 kPa).软骨细胞密度为2×107个/mL和4×107个/mL的模型中,氧分压梯度存在,但没有1×107个/mL模型中氧分压梯度变化明显.在相同细胞密度的情况下,低糖组氧分压高于无糖组. 结论关节软骨细胞-琼脂糖复合体模型内软骨细胞的氧耗量取决于培养基中的细胞密度和葡萄糖浓度环境2个方面,最佳细胞密度在1×107～2×107个/mL之间.当氧气和葡萄糖同时存在的条件下,复合体内的软骨细胞体现出消耗葡萄糖的倾向性.
목적 탐토관절연골세포-경지당복합체삼유배양모형내최리우각부분세포생장적양기화포도당조건,위체외공정화연골조직적구건제공합괄적능량대사배경. 방법근거겁보전겁법원칙,자행제작병응용침형양전겁측정1×107、2×107、4×107개/mL 3충불동세포밀도우관절연골세포-경지당체외삼유복합체모형내입체공간1～5 mm각개심도위치적양분압치,매충세포밀도모형6개,중복측량3차,분석입체공간양분압적분포정황.재상동세포밀도모형중안시부가입포도당분위무당화저당량조,측정배양액내포도당농도대모형내입체공간양분압적영향. 결과재3충세포밀도적관절연골세포-경지당복합체체외삼유배양모형1～3 mm심도위치범위내,양분압제도변화최명현,양분압치적차치고체50 mm Hg(1mm Hg=0.13 kPa).연골세포밀도위2×107개/mL화4×107개/mL적모형중,양분압제도존재,단몰유1×107개/mL모형중양분압제도변화명현.재상동세포밀도적정황하,저당조양분압고우무당조. 결론관절연골세포-경지당복합체모형내연골세포적양모량취결우배양기중적세포밀도화포도당농도배경2개방면,최가세포밀도재1×107～2×107개/mL지간.당양기화포도당동시존재적조건하,복합체내적연골세포체현출소모포도당적경향성.
Objective To investigate spatial distribution of the oxygen partial pressure (PO2) within different levels of chondrocyte-agarose constructs with various articular chondrocyte densities to find out the optimal best oxygen and glucose conditions for cell growth. Methods The local levels of PO2 and the spatial gradient of PO2 within articular chondrocyte-agarose constructs with different cell densities (1×107,2 × 107, 4 × 107 cells/mL) were measured at different depths (1 to 5 mm) . The measurements were conducted using a self-developed oxygen-sensitive electrode in 6 samples of each cell density and each measurement was repeated twice. The effects of different concentrations of glucose on the PO2 spatial distribution within the articular chondrocyte-seeded constructs with various chondrocyte densities were determined. Results The PO2 gradients were particularly evident in the top layers (1 to 3 mm) of the constructs for each of the cell densities and the difference reached up to 50 mmHg, while no obvious difference was observed in the constructs between the densities of 2 × 107 cells/mL and 4 × 107 cells/mL. The PO2 in the low glucose group was higher than in the glucose-free one at the same cell density. Conclusions Oxygen depletion is dependent on both cell density and glucose concentration in the incubating medium. The optimal cell seeding density may range from 1 × 107 to 2 × 107 cells/mL. Chondrocytes within articular chondrocyte-agarose constructs have a preference for glucose metabolism when oxygen and glucose supplies are equally abundant.