CAJ | 학술논문

目的观察瘦素(Leptin)对结肠癌HT-29细胞的数量及c-myc mRNA表达水平的影响;探讨瘦素对HT-29细胞增殖的作用及相关机制.方法体外分组培养的HT-29细胞分别加入不同浓度的瘦素作用72 h后,四甲基偶氮唑盐比色法(MTT)检测细胞生长情况;逆转录多聚酶链反应(RT-PCR)检测各组细胞c-myc mRNA表达情况.结果经不同浓度的瘦素作用后,结肠癌HT-29细胞增殖水平及c-myc mRNA表达水平较对照组均有明显提高,在一定范围内呈现浓度依赖性.结论瘦素不但能够促进结肠癌HT-29细胞的增殖,还可提高HT-29细胞c-myc基因的表达水平.瘦素提高c-myc基因表达水平的作用可能是其促癌机制之一.
목적 관찰수소(Leptin)대결장암HT-29세포적수량급c-myc mRNA표체수평적영향;탐토수소대HT-29세포증식적작용급상관궤제.방법 체외분조배양적HT-29세포분별가입불동농도적수소작용72 h후,사갑기우담서염비색법(MTT)검측세포생장정황;역전록다취매련반응(RT-PCR)검측각조세포c-myc mRNA표체정황.결과 경불동농도적수소작용후,결장암HT-29세포증식수평급c-myc mRNA표체수평교대조조균유명현제고,재일정범위내정현농도의뢰성.결론 수소불단능구촉진결장암HT-29세포적증식,환가제고HT-29세포c-myc기인적표체수평.수소제고c-myc기인표체수평적작용가능시기촉암궤제지일.
Objective To detect the effect of leptin on proliferation and c.Myc mRNA expression of human colon carcinoma HT-29 cell line and investigate the role of Leptin in the development of the HT-29 cell line.Methods Human colon carcinoma HT-29 cell line was cultured in vitro.After treatment with various concentration of Leptin for 72h.MTr was used to detect the proliferative and inhibitive status.And c-myc mRNA-expression Was detected by RT-PCR.Results After treatment with various concentration of Leptin.The cell pmlifemtion and c-myc mRNA expression Wag obviously promoted,compared with the control group.The effect wag in a time-dose-dependent manner within a certain range.Conclusion Leptin can improve cell proliferation and c-myc gene expression level in HT-29 cell line.Promoting the c-myc gene expression level may be one of the reasons that Leptin improves the proliferation of the human colon carcinoma HT-29 cell line.