中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2008年
11期
730-733
,共4页
运动训练%缺血再灌注损伤%谷氨酸%谷氨酸受体
運動訓練%缺血再灌註損傷%穀氨痠%穀氨痠受體
운동훈련%결혈재관주손상%곡안산%곡안산수체
Exercise training%Ischemia-reperfusion injury%Glutamate%Glutamate receptor
目的 研究运动训练对缺血再灌注大鼠纹状体处Ⅰ组代谢型谷氨酸受体(mGluR Ⅰ)水平变化的影响,以探讨运动训练对缺血性脑梗死的保护机制.方法 将2～3月龄雄性成年SD大鼠18只随机分为假手术组、缺血再灌注模型组(缺血再灌注组)和缺血再灌注后运动训练2周组(运动训练组),每组6只大鼠.于运动训练组运动训练2周后,3组大鼠同时断头取脑,采用逆转录-聚合酶链反应(RT-PCR)技术分析大鼠缺血部纹状体脑组织内的代谢型谷氨酸受体1型(mGluR1)和代谢型谷氨酸受体5型(mGluR5)的信使核糖核酸(mRNA)表达水平.结果 缺血再灌注组大鼠纹状体mGluR1和mGluR5的mRNA升高明显(P<0.01),运动训练组大鼠纹状体mGluR1和mGluR5的mRNA较缺血再灌注组有明显下调(P<0.01).结论 运动训练可以使缺血再灌注大鼠Ⅰ组代谢型谷氨酸受体(mGluR Ⅰ)水平下调,这可能是抑制兴奋毒性谷氨酸的产生的重要机制之一.
目的 研究運動訓練對缺血再灌註大鼠紋狀體處Ⅰ組代謝型穀氨痠受體(mGluR Ⅰ)水平變化的影響,以探討運動訓練對缺血性腦梗死的保護機製.方法 將2～3月齡雄性成年SD大鼠18隻隨機分為假手術組、缺血再灌註模型組(缺血再灌註組)和缺血再灌註後運動訓練2週組(運動訓練組),每組6隻大鼠.于運動訓練組運動訓練2週後,3組大鼠同時斷頭取腦,採用逆轉錄-聚閤酶鏈反應(RT-PCR)技術分析大鼠缺血部紋狀體腦組織內的代謝型穀氨痠受體1型(mGluR1)和代謝型穀氨痠受體5型(mGluR5)的信使覈糖覈痠(mRNA)錶達水平.結果 缺血再灌註組大鼠紋狀體mGluR1和mGluR5的mRNA升高明顯(P<0.01),運動訓練組大鼠紋狀體mGluR1和mGluR5的mRNA較缺血再灌註組有明顯下調(P<0.01).結論 運動訓練可以使缺血再灌註大鼠Ⅰ組代謝型穀氨痠受體(mGluR Ⅰ)水平下調,這可能是抑製興奮毒性穀氨痠的產生的重要機製之一.
목적 연구운동훈련대결혈재관주대서문상체처Ⅰ조대사형곡안산수체(mGluR Ⅰ)수평변화적영향,이탐토운동훈련대결혈성뇌경사적보호궤제.방법 장2～3월령웅성성년SD대서18지수궤분위가수술조、결혈재관주모형조(결혈재관주조)화결혈재관주후운동훈련2주조(운동훈련조),매조6지대서.우운동훈련조운동훈련2주후,3조대서동시단두취뇌,채용역전록-취합매련반응(RT-PCR)기술분석대서결혈부문상체뇌조직내적대사형곡안산수체1형(mGluR1)화대사형곡안산수체5형(mGluR5)적신사핵당핵산(mRNA)표체수평.결과 결혈재관주조대서문상체mGluR1화mGluR5적mRNA승고명현(P<0.01),운동훈련조대서문상체mGluR1화mGluR5적mRNA교결혈재관주조유명현하조(P<0.01).결론 운동훈련가이사결혈재관주대서Ⅰ조대사형곡안산수체(mGluR Ⅰ)수평하조,저가능시억제흥강독성곡안산적산생적중요궤제지일.
Objective To observe the effect of treadmill training on metabotropic glutamate receptor of a middle cerebral artery occlusion model in rat and its underlying mechanisms. Methods Sprague-Dawley male rats of 2 or 3 months old were randomly divided into 3 groups: a sham operation group, an iscbemia-reperfnsion group and a treadmill training group (subject to 2 weeks of training after the ischemia-reperfusion). All the animals were sacri-ficed after 2 weeks of training and their brains were sampled for measurement of the expression level of the striatum mGluR Ⅰ-mRNA, using RT-PCR technique. Results The striatum mRNA of mGluR1 and mGluR5 was signifi-cantly elevated in the ischemia-reperfusion group(P<0.01), but treadmill training significantly suppressed the ele-vation of the expression of mRNA of mGluR1 and mGluR5 (P<0.01). Conclusions Treadmill training can signif-icantly downregulate mGluR Ⅰ-mRNA expression. This might be one of the important mechanisms for inhibition of the excitatory glutamate production.
