CAJ | 학술논문

目的建立防护面具高效过滤罐微生物气溶胶测试评价方法,对过滤罐的实际防护效果进行测试评价.方法 Serratia marcescens作为模式细菌繁殖体气溶胶、Bacillus subtilis var niger芽孢作为模式芽孢气溶胶、噬菌体f2作为模式病毒气溶胶,使用实验室建立的微生物气溶胶检测技术平台,人工发生模式微生物气溶胶,分别在过滤罐过滤前后使用空气微生物采样器进行定量采样,根据过滤前后模式微生物气溶胶的浓度分别计算细菌、芽孢、病毒气溶胶过滤效率.1-1、1-2、1-3、1-44个只含有高效过滤材料的过滤罐分别测试了Serratia marcescens、Bacillus subtilis var niger、噬菌体f2气溶胶的过滤效率.543、544 2个装有活性炭的高效过滤罐测试了对Scrratia marcescens气溶胶的过滤效率.结果 1-1、1-2、1-3 3个高效过滤罐对Serratia marcescens、Bacillus subtilis var niger芽孢、噬菌体f2的气溶胶的过滤效率为100.000%,1-4高效过滤罐对Bacillus subtilis var niger芽孢气溶胶的过滤效率为990997%、Serratia marcescerts和噬菌体f2气溶胶的过滤效率均为100.000%.加入活性炭后543、544 2个过滤罐对Serratia marcescens气溶胶的过滤效率均为100.000%.结论建立的检测方法可以用于高效过滤罐微生物气溶胶防护效果的评价,高效过滤罐(包括装有活性炭者)微生物气溶胶防护效果均佳.
목적 건립방호면구고효과려관미생물기용효측시평개방법,대과려관적실제방호효과진행측시평개.방법 Serratia marcescens작위모식세균번식체기용효、Bacillus subtilis var niger아포작위모식아포기용효、서균체f2작위모식병독기용효,사용실험실건립적미생물기용효검측기술평태,인공발생모식미생물기용효,분별재과려관과려전후사용공기미생물채양기진행정량채양,근거과려전후모식미생물기용효적농도분별계산세균、아포、병독기용효과려효솔.1-1、1-2、1-3、1-44개지함유고효과려재료적과려관분별측시료Serratia marcescens、Bacillus subtilis var niger、서균체f2기용효적과려효솔.543、544 2개장유활성탄적고효과려관측시료대Scrratia marcescens기용효적과려효솔.결과 1-1、1-2、1-3 3개고효과려관대Serratia marcescens、Bacillus subtilis var niger아포、서균체f2적기용효적과려효솔위100.000%,1-4고효과려관대Bacillus subtilis var niger아포기용효적과려효솔위990997%、Serratia marcescerts화서균체f2기용효적과려효솔균위100.000%.가입활성탄후543、544 2개과려관대Serratia marcescens기용효적과려효솔균위100.000%.결론 건립적검측방법가이용우고효과려관미생물기용효방호효과적평개,고효과려관(포괄장유활성탄자)미생물기용효방호효과균가.
Objective To establish a testing and evaluating method for filtration efficiency of the eanister against microbial aerosol. Methods Serratia marcescens aerosol served as model of bacterial aerosol, Bacillus subtilis var niger aerosol as model of spores aerosol, bacteriophage f2 aerosol as model of viral aerosol. Employing the microbial aerosol testing platform was established in lab, models of microbial aerosol generated artificially were sampled quantitatively by air samplers before and after filtrating by canisters, respectively. Filtration efficiency was determined by the concentration of microbial aerosol in the air sample before and after filtrating. The four canisters of 1-1,1-2, 1-3,1-4 were tested for the filtration efficiency against &rratia marcescens ,Bacillus subtilis var niger and phage f2 aerosol The two canisters of 543 and 544 canisters equipped with active carbon were tested for the fdtration efficiencies against Serratia marcescens aerosol. Results The filtration efficiency of 1-1, 1-2, 1-3 canisters against Serratia marcescens, Bacillus subtilis var niger and phage f2 aerosol was 100.000%. The filtration efficiency of 1-4 canister filtration efficiency against Bacillus subtilis var niger spores aerosol was 99. 997% and efficiency of the other two aerosol was 100. 000%. The filtration efficiency of the two canisters of 543 and 544 to those attached with active carbon against Serrutia marcesccns aerosol was 100. 000%. Conclusion The testing method might be used to evaluate the protective performance of the canister against microbiological aerosol. The effect of the canisters( including those equipped with active carbon) against microbiological aerosol should be reliable.