中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2010年
1期
20-24
,共5页
张英%倪安平%崔京涛%李倩%窦亚玲%吴叶丽%王健伟
張英%倪安平%崔京濤%李倩%竇亞玲%吳葉麗%王健偉
장영%예안평%최경도%리천%두아령%오협려%왕건위
正黏病毒科%菌斑试验%微生物敏感性试验%奥司他韦%金刚烷胺%利巴韦林%板蓝根
正黏病毒科%菌斑試驗%微生物敏感性試驗%奧司他韋%金剛烷胺%利巴韋林%闆藍根
정점병독과%균반시험%미생물민감성시험%오사타위%금강완알%리파위림%판람근
Orthomyxoviridae%Plaque assay%Microbial sensitivity tests%Oseltamivir%Amantadine%Ribavirin%Badix isatidis
目的 建立PRA以评估奥司他韦(达菲)、金刚烷胺、利巴韦林和板蓝根4种药物的体外抗流感病毒作用.方法 建立PRA,选取临床分离的流感病毒株8株,其中甲型流感病毒3株,乙型流感病毒5株.病毒培养并接种,利用PRA测定奥司他韦、金刚烷胺、利巴韦林和板蓝根4种药物体外对流感病毒的IC_(50)值.结果 8株甲、乙型流感病毒临床分离株的PRA测定结果显示,奥司他韦对甲型流感病毒IC_(50)值为0.064-0.128 mg/L,金刚烷胺为0.5 mg/L,利巴韦林对甲型流感病毒不敏感(IC_(50)>8 mg/L),板蓝根无抗病毒作用;奥司他韦、金刚烷胺、利巴韦林和板蓝根对乙型流感病毒均未发现体外抗病毒作用.结论 奥司他韦和金刚烷胺对甲型流感病毒敏感,利巴韦林不敏感,板蓝根无抗病毒作用,该4种药物未发现体外抗乙型流感病毒作用.
目的 建立PRA以評估奧司他韋(達菲)、金剛烷胺、利巴韋林和闆藍根4種藥物的體外抗流感病毒作用.方法 建立PRA,選取臨床分離的流感病毒株8株,其中甲型流感病毒3株,乙型流感病毒5株.病毒培養併接種,利用PRA測定奧司他韋、金剛烷胺、利巴韋林和闆藍根4種藥物體外對流感病毒的IC_(50)值.結果 8株甲、乙型流感病毒臨床分離株的PRA測定結果顯示,奧司他韋對甲型流感病毒IC_(50)值為0.064-0.128 mg/L,金剛烷胺為0.5 mg/L,利巴韋林對甲型流感病毒不敏感(IC_(50)>8 mg/L),闆藍根無抗病毒作用;奧司他韋、金剛烷胺、利巴韋林和闆藍根對乙型流感病毒均未髮現體外抗病毒作用.結論 奧司他韋和金剛烷胺對甲型流感病毒敏感,利巴韋林不敏感,闆藍根無抗病毒作用,該4種藥物未髮現體外抗乙型流感病毒作用.
목적 건립PRA이평고오사타위(체비)、금강완알、리파위림화판람근4충약물적체외항류감병독작용.방법 건립PRA,선취림상분리적류감병독주8주,기중갑형류감병독3주,을형류감병독5주.병독배양병접충,이용PRA측정오사타위、금강완알、리파위림화판람근4충약물체외대류감병독적IC_(50)치.결과 8주갑、을형류감병독림상분리주적PRA측정결과현시,오사타위대갑형류감병독IC_(50)치위0.064-0.128 mg/L,금강완알위0.5 mg/L,리파위림대갑형류감병독불민감(IC_(50)>8 mg/L),판람근무항병독작용;오사타위、금강완알、리파위림화판람근대을형류감병독균미발현체외항병독작용.결론 오사타위화금강완알대갑형류감병독민감,리파위림불민감,판람근무항병독작용,해4충약물미발현체외항을형류감병독작용.
Objective To establish plaque reduction assay and evaluate the activities of oseltamivir (tamiflu),amantadine,ribavirin and herb radix isatidis against influenza virus in vitro.Methods Plaque reduction assay was used to determine IC_(50) values of four studied drugs above in this susceptibility testing in which 8 clinical isolates(three influenza A virus isolates and five influenza B virus isolateds)were inoculated and tested.Results By testing of 8 clinical isolates of influenza virus A and B isolated between the year 2001 to 2008,oseltamivir and amantadine were found to be sensitive to influenza A virus with IC_(50) of 0.064 -0.128 mg/L and 0.5 mg/L,respectively.However,ribavirin(IC_(50)>8 mg/L)was not found to be sensitive,and herb radix isatidis had totally no activities.Unfortunately.all four studied drugs were not found to have activities against influenza B virus in vitro.Conclusions It Was indicated that oseltamivir and amantadine.but not ribavirin and herb radix isatidis.are sensitive to influenza A virus.All four studied drugs were not found to have activities against influenza B virus in vitro.