CAJ | 학술논문

目的建立PRA以评估奥司他韦(达菲)、金刚烷胺、利巴韦林和板蓝根4种药物的体外抗流感病毒作用.方法建立PRA,选取临床分离的流感病毒株8株,其中甲型流感病毒3株,乙型流感病毒5株.病毒培养并接种,利用PRA测定奥司他韦、金刚烷胺、利巴韦林和板蓝根4种药物体外对流感病毒的IC_(50)值.结果 8株甲、乙型流感病毒临床分离株的PRA测定结果显示,奥司他韦对甲型流感病毒IC_(50)值为0.064-0.128 mg/L,金刚烷胺为0.5 mg/L,利巴韦林对甲型流感病毒不敏感(IC_(50)>8 mg/L),板蓝根无抗病毒作用;奥司他韦、金刚烷胺、利巴韦林和板蓝根对乙型流感病毒均未发现体外抗病毒作用.结论奥司他韦和金刚烷胺对甲型流感病毒敏感,利巴韦林不敏感,板蓝根无抗病毒作用,该4种药物未发现体外抗乙型流感病毒作用.
목적 건립PRA이평고오사타위(체비)、금강완알、리파위림화판람근4충약물적체외항류감병독작용.방법 건립PRA,선취림상분리적류감병독주8주,기중갑형류감병독3주,을형류감병독5주.병독배양병접충,이용PRA측정오사타위、금강완알、리파위림화판람근4충약물체외대류감병독적IC_(50)치.결과 8주갑、을형류감병독림상분리주적PRA측정결과현시,오사타위대갑형류감병독IC_(50)치위0.064-0.128 mg/L,금강완알위0.5 mg/L,리파위림대갑형류감병독불민감(IC_(50)>8 mg/L),판람근무항병독작용;오사타위、금강완알、리파위림화판람근대을형류감병독균미발현체외항병독작용.결론 오사타위화금강완알대갑형류감병독민감,리파위림불민감,판람근무항병독작용,해4충약물미발현체외항을형류감병독작용.
Objective To establish plaque reduction assay and evaluate the activities of oseltamivir (tamiflu),amantadine,ribavirin and herb radix isatidis against influenza virus in vitro.Methods Plaque reduction assay was used to determine IC_(50) values of four studied drugs above in this susceptibility testing in which 8 clinical isolates(three influenza A virus isolates and five influenza B virus isolateds)were inoculated and tested.Results By testing of 8 clinical isolates of influenza virus A and B isolated between the year 2001 to 2008,oseltamivir and amantadine were found to be sensitive to influenza A virus with IC_(50) of 0.064 -0.128 mg/L and 0.5 mg/L,respectively.However,ribavirin(IC_(50)>8 mg/L)was not found to be sensitive,and herb radix isatidis had totally no activities.Unfortunately.all four studied drugs were not found to have activities against influenza B virus in vitro.Conclusions It Was indicated that oseltamivir and amantadine.but not ribavirin and herb radix isatidis.are sensitive to influenza A virus.All four studied drugs were not found to have activities against influenza B virus in vitro.