中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2004年
36期
8418-8420
,共3页
刘志诚%孙凤岷%袁锦虹%姜军作%吕雅妮%衣运玲
劉誌誠%孫鳳岷%袁錦虹%薑軍作%呂雅妮%衣運玲
류지성%손봉민%원금홍%강군작%려아니%의운령
肥胖症/病理生理学%纹状体/针灸效应%体重%小鼠,肥胖%一氧化氮/代谢%一氧化氮合酶/代谢%针刺疗法
肥胖癥/病理生理學%紋狀體/針灸效應%體重%小鼠,肥胖%一氧化氮/代謝%一氧化氮閤酶/代謝%針刺療法
비반증/병리생이학%문상체/침구효응%체중%소서,비반%일양화담/대사%일양화담합매/대사%침자요법
背景:已知针灸减肥机制涉及神经及神经体液调节.考虑到纹状体参与了对下丘脑摄食中枢、植物神经中枢、体温调节中枢等的调控.针刺对肥胖大鼠纹状体组织一氧化氮含量和一氧化氮合酶(nitric oxide syn-thase,NOS)活性的调整作用如何?目的:探讨针刺对肥胖大鼠纹状体组织一氧化氮和NOS含量的影响,进一步深化针刺减肥的中枢神经作用机制.设计:以自身对照、相互对照为主的实验研究.单位:南京中医药大学第二临床医学院针灸研究所、南京人口管理学院生殖医学教研室.材料:本实验在南京中医药大学第二临床医学院针灸研究所完成.1月龄刚断乳SD雄性大鼠,体质量50～70 g,由南京军区总医院实验动物中心提供,动物级别为清洁级.干预:以普通全价鼠饲料喂养的大鼠作为正常组,将造模成功的实验性肥胖大鼠随机分为对照组和针刺组,每组6只.针刺组大鼠给予针刺治疗14 d,正常组和对照组大鼠分别每天放入大鼠固定器中适应15 min,持续14 d.采用神经生化技术观察针刺治疗前后肥胖大鼠体质量、体脂及纹状体组织一氧化氮和NOS含量的变化.主要观察指标:①针刺对实验性肥胖大鼠肥胖指标及对心包、肾周和附睾脂肪量的影响.②针刺对各组大鼠纹状体组织一氧化氮含量和一氧化氮合酶活性的影响.③大鼠纹状体组织一氧化氮含量和NOS活性与体质量和Lee's指数的相关性.结果:肥胖大鼠体质量和体脂均显著高于正常大鼠水平,差异均有显著性意义(t=8.86,9.99,8.31,P均＜0.01);纹状体组织一氧化氮和NOS水平均显著低于正常大鼠水平,差异均有显著性意义(t=5.48,4.37,P均＜0.01);大鼠纹状体组织一氧化氮和NOS水平与体质量和Lee's指数呈负相关(r=-0.78,-0.71;P＜0.01).针刺治疗能使肥胖大鼠纹状体组织一氧化氮和NOS水平明显回升,与针刺前比较,差异均有显著性意义(t=3.74,3.48,P均＜0.01).结论:纹状体组织一氧化氮和NOS水平异常可能是肥胖发病的因素;针刺对肥胖机体纹状体组织一氧化氮和NOS水平的良性调整作用可能是实现减肥效应的中枢作用机制之一.
揹景:已知針灸減肥機製涉及神經及神經體液調節.攷慮到紋狀體參與瞭對下丘腦攝食中樞、植物神經中樞、體溫調節中樞等的調控.針刺對肥胖大鼠紋狀體組織一氧化氮含量和一氧化氮閤酶(nitric oxide syn-thase,NOS)活性的調整作用如何?目的:探討針刺對肥胖大鼠紋狀體組織一氧化氮和NOS含量的影響,進一步深化針刺減肥的中樞神經作用機製.設計:以自身對照、相互對照為主的實驗研究.單位:南京中醫藥大學第二臨床醫學院針灸研究所、南京人口管理學院生殖醫學教研室.材料:本實驗在南京中醫藥大學第二臨床醫學院針灸研究所完成.1月齡剛斷乳SD雄性大鼠,體質量50～70 g,由南京軍區總醫院實驗動物中心提供,動物級彆為清潔級.榦預:以普通全價鼠飼料餵養的大鼠作為正常組,將造模成功的實驗性肥胖大鼠隨機分為對照組和針刺組,每組6隻.針刺組大鼠給予針刺治療14 d,正常組和對照組大鼠分彆每天放入大鼠固定器中適應15 min,持續14 d.採用神經生化技術觀察針刺治療前後肥胖大鼠體質量、體脂及紋狀體組織一氧化氮和NOS含量的變化.主要觀察指標:①針刺對實驗性肥胖大鼠肥胖指標及對心包、腎週和附睪脂肪量的影響.②針刺對各組大鼠紋狀體組織一氧化氮含量和一氧化氮閤酶活性的影響.③大鼠紋狀體組織一氧化氮含量和NOS活性與體質量和Lee's指數的相關性.結果:肥胖大鼠體質量和體脂均顯著高于正常大鼠水平,差異均有顯著性意義(t=8.86,9.99,8.31,P均＜0.01);紋狀體組織一氧化氮和NOS水平均顯著低于正常大鼠水平,差異均有顯著性意義(t=5.48,4.37,P均＜0.01);大鼠紋狀體組織一氧化氮和NOS水平與體質量和Lee's指數呈負相關(r=-0.78,-0.71;P＜0.01).針刺治療能使肥胖大鼠紋狀體組織一氧化氮和NOS水平明顯迴升,與針刺前比較,差異均有顯著性意義(t=3.74,3.48,P均＜0.01).結論:紋狀體組織一氧化氮和NOS水平異常可能是肥胖髮病的因素;針刺對肥胖機體紋狀體組織一氧化氮和NOS水平的良性調整作用可能是實現減肥效應的中樞作用機製之一.
배경:이지침구감비궤제섭급신경급신경체액조절.고필도문상체삼여료대하구뇌섭식중추、식물신경중추、체온조절중추등적조공.침자대비반대서문상체조직일양화담함량화일양화담합매(nitric oxide syn-thase,NOS)활성적조정작용여하?목적:탐토침자대비반대서문상체조직일양화담화NOS함량적영향,진일보심화침자감비적중추신경작용궤제.설계:이자신대조、상호대조위주적실험연구.단위:남경중의약대학제이림상의학원침구연구소、남경인구관이학원생식의학교연실.재료:본실험재남경중의약대학제이림상의학원침구연구소완성.1월령강단유SD웅성대서,체질량50～70 g,유남경군구총의원실험동물중심제공,동물급별위청길급.간예:이보통전개서사료위양적대서작위정상조,장조모성공적실험성비반대서수궤분위대조조화침자조,매조6지.침자조대서급여침자치료14 d,정상조화대조조대서분별매천방입대서고정기중괄응15 min,지속14 d.채용신경생화기술관찰침자치료전후비반대서체질량、체지급문상체조직일양화담화NOS함량적변화.주요관찰지표:①침자대실험성비반대서비반지표급대심포、신주화부고지방량적영향.②침자대각조대서문상체조직일양화담함량화일양화담합매활성적영향.③대서문상체조직일양화담함량화NOS활성여체질량화Lee's지수적상관성.결과:비반대서체질량화체지균현저고우정상대서수평,차이균유현저성의의(t=8.86,9.99,8.31,P균＜0.01);문상체조직일양화담화NOS수평균현저저우정상대서수평,차이균유현저성의의(t=5.48,4.37,P균＜0.01);대서문상체조직일양화담화NOS수평여체질량화Lee's지수정부상관(r=-0.78,-0.71;P＜0.01).침자치료능사비반대서문상체조직일양화담화NOS수평명현회승,여침자전비교,차이균유현저성의의(t=3.74,3.48,P균＜0.01).결론:문상체조직일양화담화NOS수평이상가능시비반발병적인소;침자대비반궤체문상체조직일양화담화NOS수평적량성조정작용가능시실현감비효응적중추작용궤제지일.
BACKGROUND: It is known that the mechanism of acupuncture in treating obesity comes up with the neural and neurohumoral regulations. Striatum participates in regulating and controlling food taking centrum, plant nerve centrum and animal heat centrum of hypothalamus. What is the regulating effect of acupuncture on nitric oxide (NO) content and nitric oxide synthase (NOS) activity in striatum tissues?OBJECTIVE: To study the effects of acupuncture on the content of NO and NOS in the striatum tissue of obese rats, and further investigate the mechanism of acupuncture effect on reducing body mass in central nervous system.DESIGN: Self-controlled and mutual controlled experiment.SETTING: Acupuncture Institute of Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine, and Department of Reproduction Medicine, Nanjing College for Population Programmed Management.PARTICIPANTS: This experiment was carried out in the Acupuncture Institute of Second Clinical Medical College. One-month old male SD rats just in ablactation, weighing 50 to 70 g, were provided by the Animal Experiment Center of General Hospital of Nanjing Military Area Command , and the animals were in the clean grade.INTERVENTIONS: Rats fed with ordinary diet were in the normal group. Experimental obese rats models were randomly divided as control group and acupuncture group, 6 in each. Rats in the acupuncture group were given acupuncture treatment for 14 days, and rats in the normal and control groups were put into rat fixation-machine for 15 minutes everyday, lasting for 14 days. Body mass, body lipid and contents of NO and NOS in striatum tissues of the obese rats were observed with nervous biochemical technology before and after acupuncture respectively.MAIN OUTCOME MEASURES: ① Effect of acupuncture on obesity index, fat contents of pericardium, kidney and epididymis of obese rats. ②Effect of acupuncture on NO and NOS contents in striatum tissue of obese rats. ③ Correlation of NO content and NOS activity with body mass and Lee's index in striatum tissue of obese rats.RESULTS: The body mass and body fat content of the obese rats were all significantly higher than those in the normal rats( t = 8.86, 9.99, 8.31, P ＜ 0.01); NO content and NOS activity in the striatum tissue of the obese rats were all markedly higher than those in the normal rats( t =5.48, 4.37,P ＜ 0.01) . NO content and NOS activity were highly negatively correlated with body mass and Lee' s index(r= -0.78, -0.71, P ＜ 0.01) .Acupuncture significantly increased the NO content and NOS activity in the striatum tissue of the obese rats as compared with those before acupuncture(t=3.74, 3.48, P ＜0.01).CONCLUSION: The abnormal levels of NO and NOS in striatum tissue might be an important factor to cause simple obesity. The good regulation of acupuncture on the levels of NO and NOS in striatum tissue might be one of the central nervous mechanisms of reducing body mass in obese organisms.
