CAJ | 학술논문

为探讨金属离子对法夫酵母生物合成虾青素的影响,采用Plackett-Burman设计从八种金属离子中筛选出了对虾青素合成有显著影响的四种金属离子,即Zn2+、Cu2+、Mn2+、Fe3+.在此基础上采用最陡爬坡实验逼近最优区域,Box-Behnken设计对其浓度进行优化,结果表明:最佳离子浓度分别为1.45、1.94、1.3、0.89 μmol/L,虾青素产量达6.89 mg/L,较优化前提高了51%左右.
위탐토금속리자대법부효모생물합성하청소적영향,채용Plackett-Burman설계종팔충금속리자중사선출료대하청소합성유현저영향적사충금속리자,즉Zn2+、Cu2+、Mn2+、Fe3+.재차기출상채용최두파파실험핍근최우구역,Box-Behnken설계대기농도진행우화,결과표명:최가리자농도분별위1.45、1.94、1.3、0.89 μmol/L,하청소산량체6.89 mg/L,교우화전제고료51%좌우.
The effects of metal ions on astaxanthin accumulation of Phaffia rhodozyma were investigated in the paper. Three types of experimental designs were used to optimize the metal ions conditions for astaxanthin production. The first Plackett-Burman experiment design was adopted to screen key factors from eight ions. The results indicated that Zn2+, Cu2+, Mn2+ and Fe3+ contribute to synthesize astaxanthin greatly. With this first design the most important factors were determined to be used in the second experimental strategy. The method of steepest ascent was applied in order to rapidly approach the optimum.Finally, the optimal concentrations of Zn2+, Cu2+, Mn2+ and Fe3+ are 1.45 μmol/L, 1.94 μmol/L, 1.3 μmol/L and 0.89 μmol/L respectively, optimized through Box-Behnken design and RSM. In optimum conditions, the yield of astaxanthin is about 6.89mg/L, 51% higher than the production under the initial conditions.