小干涉RNA质粒抑制Fn14表达对肝癌细胞增殖的影响
소간섭RNA질립억제Fn14표체대간암세포증식적영향
The Effect of siRNA Vector Inhibiting Fn14 Expression on Hepatoma Cell Proliferation
  • 万方数据
    西南军医 서남군의
    JOURNAL OF MILITARY SURGEON IN SOUTHWEST CHINA
    2008年 3期 7-9 ,共3页

    程晓刚%叶琬%邹强%陈玮%金虹%徐艳%张绍兰%冷言冰%李晋川

    정효강%협완%추강%진위%금홍%서염%장소란%랭언빙%리진천

    Fn14%RNA干涉%肝癌细胞%增殖 Fn14%RNA榦涉%肝癌細胞%增殖
    Fn14%RNA간섭%간암세포%증식
    目的 观察成纤维细胞生长因子诱导的早期反应蛋白14(Fibroblast growth factor-inducible immediate-early response protein 14、Fn14)表达对肝癌细胞增殖的影响.方法 构建PAVU6- Fn14 siRNA干涉质粒并将其转染肝癌细胞株QGY7703,MTT法检测抑制Fn14蛋白表达对肝癌细胞增殖的影响,在培养体系加入肿瘤坏死因子相关弱凋亡诱导因子(Tumor necrosis factor related weak inducer of apoptosis、TWEAK)蛋白后检测肝癌细胞增殖活性.结果 成功构建PAVU6- Fn14 siRNA干涉质粒,转化肝癌细胞后肝癌细胞增殖受抑,培养体系加入TWEAK蛋白后肝癌细胞增殖活性仍受抑.结论 Fn14蛋白表达降低可抑制肝癌细胞的增殖活性,应用RNA干涉技术抑制Fn14表达对肝癌细胞增殖具抑制作用.
    목적 관찰성섬유세포생장인자유도적조기반응단백14(Fibroblast growth factor-inducible immediate-early response protein 14、Fn14)표체대간암세포증식적영향.방법 구건PAVU6- Fn14 siRNA간섭질립병장기전염간암세포주QGY7703,MTT법검측억제Fn14단백표체대간암세포증식적영향,재배양체계가입종류배사인자상관약조망유도인자(Tumor necrosis factor related weak inducer of apoptosis、TWEAK)단백후검측간암세포증식활성.결과 성공구건PAVU6- Fn14 siRNA간섭질립,전화간암세포후간암세포증식수억,배양체계가입TWEAK단백후간암세포증식활성잉수억.결론 Fn14단백표체강저가억제간암세포적증식활성,응용RNA간섭기술억제Fn14표체대간암세포증식구억제작용.
    원문보기 원문다운로드 사이트로이동
저자의 최근 논문