军事医学科学院院刊
軍事醫學科學院院刊
군사의학과학원원간
BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES
2010年
1期
40-42,50
,共4页
高胰岛素-正葡萄糖钳夹技术%药效学%比格犬
高胰島素-正葡萄糖鉗夾技術%藥效學%比格犬
고이도소-정포도당겸협기술%약효학%비격견
euglycemic clamp technique%pharmacodynamics%beagle dogs
目的 在比格犬中建立高胰岛素-正葡萄糖钳夹技术.方法 以5只健康比格犬为实验对象进行高胰岛素-正葡萄糖钳夹实验,考察血糖、胰岛素、C肽及胰高血糖素等指标.结果 成功将血糖控制在目标水平,变异系数小于5%;实验过程中血清胰岛素最后稳定在(40.0±3.8)mIU/L,显著高于基础胰岛素水平;血清C肽浓度显示内源性胰岛素受到了明显的抑制,胰高血糖素没有显著升高.结论 在比格犬体内建立高胰岛素-正葡萄糖钳夹技术,并将其成功用于胰岛素制剂药效学的评价.
目的 在比格犬中建立高胰島素-正葡萄糖鉗夾技術.方法 以5隻健康比格犬為實驗對象進行高胰島素-正葡萄糖鉗夾實驗,攷察血糖、胰島素、C肽及胰高血糖素等指標.結果 成功將血糖控製在目標水平,變異繫數小于5%;實驗過程中血清胰島素最後穩定在(40.0±3.8)mIU/L,顯著高于基礎胰島素水平;血清C肽濃度顯示內源性胰島素受到瞭明顯的抑製,胰高血糖素沒有顯著升高.結論 在比格犬體內建立高胰島素-正葡萄糖鉗夾技術,併將其成功用于胰島素製劑藥效學的評價.
목적 재비격견중건립고이도소-정포도당겸협기술.방법 이5지건강비격견위실험대상진행고이도소-정포도당겸협실험,고찰혈당、이도소、C태급이고혈당소등지표.결과 성공장혈당공제재목표수평,변이계수소우5%;실험과정중혈청이도소최후은정재(40.0±3.8)mIU/L,현저고우기출이도소수평;혈청C태농도현시내원성이도소수도료명현적억제,이고혈당소몰유현저승고.결론 재비격견체내건립고이도소-정포도당겸협기술,병장기성공용우이도소제제약효학적평개.
Objective To establish a euglycemic clamp technique in beagle dogs. Methods The euglycemic clamp technique was applied in healthy beagle dogs and the blood glucose, insulin, C-peptide, insulin and glucagon were monitored during the clamp. Results The blood glucose was controlled within the basal level.The coefficient of variation was less than 5% during the clamp. The serum insulin concentration finally reached(40.0±3.8)mIU/L stably and a significant inhibition was shown in endogenous insulin by the determination of C-peptide. But there was no significant increase in serum glucagon compared with basal values.Conclusion Methodology confirmed that the euglycemic clamp technique is successful in beagle dogs and can be applied in the study of pharmacodynamics of insulin preparations.
