CAJ | 학술논문

用RT-PCR方法对山东省猪瘟病毒(CSFV)流行毒株的E2基因进行克隆,用DNAstar软件对获得的CSFV及已发表的CSFV毒株E2基因核苷酸和氨基酸序列进行了比较和分析,构建了CSFV遗传发生树.结果表明:扩增的12株猪瘟流行病毒的E2基因长度均为270 bp,与预期大小一致;12株病毒株均属于1个组群中的2个亚群,其中,病毒株SDTA-1、SDTA-2属于同一个亚群,E2基因与疫苗株HCLV相比,核苷酸、氨基酸同源性分别为83.5 %、88.9 %,其余10株病毒株属于同一个亚群,E2基因与疫苗株HCLV相比,核苷酸、氨基酸同源性分别为80.5 %～83.5 %、82.2 %～84.4 %.
용RT-PCR방법대산동성저온병독(CSFV)류행독주적E2기인진행극륭,용DNAstar연건대획득적CSFV급이발표적CSFV독주E2기인핵감산화안기산서렬진행료비교화분석,구건료CSFV유전발생수.결과표명:확증적12주저온류행병독적E2기인장도균위270 bp,여예기대소일치;12주병독주균속우1개조군중적2개아군,기중,병독주SDTA-1、SDTA-2속우동일개아군,E2기인여역묘주HCLV상비,핵감산、안기산동원성분별위83.5 %、88.9 %,기여10주병독주속우동일개아군,E2기인여역묘주HCLV상비,핵감산、안기산동원성분별위80.5 %～83.5 %、82.2 %～84.4 %.
The major antigen coding region sequences of E2 gene of twelve recent prevalent field classical swine fever viruses(CSFV) collected from Shandong province were obtained with reverse trancriptase polymerse chain reaction (RT-PCR) and sequencing. The abovementioned sequences were analysed with DNAstar and compared with the previously published sequences of references strains. The phylogenetic tree was constructed. The results showed that all the twelve prevalent CSFV strains belonged to gene group 2 which was divided into 2 subgroups. 10 of the 12 strains were in subgroup 1 while the other 2 strains in subgroup 2. The identities of nucleotide sequence of the two subgroups was 80.5 %～83.5 %. These findings revealed that recent prevalent CSFV strains in Shandong province had been evolving towards the direction far from the C strain vaccine virus.