CAJ | 학술논문

利用前期获得并经基因工程改造的一株高效几丁质酶降解菌进行发酵产酶法降解几丁质胶体与膜分离相耦合制备几丁寡糖的中试研究.经过中试发酵工艺、酶反应工艺、分离纯化工艺的研究,最终确定了一条高效制备几丁寡糖的中试规模工艺流程,该流程中反应分离一体化,过程实现连续操作,降解酶可以连续使用,提高了收率,降低了成本,有利于实现产业化.通过该流程制备的几丁寡糖混合物含几丁2、3、4糖分别为71.3%、23.5%、2.4%.
이용전기획득병경기인공정개조적일주고효궤정질매강해균진행발효산매법강해궤정질효체여막분리상우합제비궤정과당적중시연구.경과중시발효공예、매반응공예、분리순화공예적연구,최종학정료일조고효제비궤정과당적중시규모공예류정,해류정중반응분리일체화,과정실현련속조작,강해매가이련속사용,제고료수솔,강저료성본,유리우실현산업화.통과해류정제비적궤정과당혼합물함궤정2、3、4당분별위71.3%、23.5%、2.4%.
Chitin oligosaccharides was produced by chitinases digestion of chitin colloid coupling with membrane separation.The chitinases was produced by fermentation through wild ( aeromonas caviae CB101 ) bacteria changed by our group.A method suitable for industrialization was suggested through detail fermentation,enzyme digestion,and separation study.The character of this method is digestion and separation can be operated at same time in series.The purity of N-Acetylchitooligosaccharides (dimer,trimer,tetramer) produced by this method is 71.3%,23.5%,2.4%.Chitinases can be used more effectively and with high yield and low cost in favor the development of industrialization.