中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
3期
139-142
,共4页
谷甸娜%陈永平%张磊%张晓华%卢洁%郑毅%郑明华
穀甸娜%陳永平%張磊%張曉華%盧潔%鄭毅%鄭明華
곡전나%진영평%장뢰%장효화%로길%정의%정명화
CD4阳性T淋巴细胞%白细胞介素2受体α亚单位%肝硬化%四氯化碳%疾病模型,动物
CD4暘性T淋巴細胞%白細胞介素2受體α亞單位%肝硬化%四氯化碳%疾病模型,動物
CD4양성T림파세포%백세포개소2수체α아단위%간경화%사록화탄%질병모형,동물
CD4-positive T-lymphocytes Interleukin-2 receptor alpha subunit%Liver cirrhosis%Carbon tetrachloride%Disease models,animal
目的 探讨CD4+CD25+调节性T淋巴细胞(Tregs)在肝纤维化免疫发病机制中的作用.方法 26只大鼠分为对照组(6只)和模型组(20只),通过皮下注射四氯化碳(CCl4)建立肝纤维化模型,检测血清肝功能和纤维化指标,观察肝脏病理学变化,流式细胞仪测定外周血及脾脏CD4+CD25+Tregs数量的变化.两组间差异比较采用t检验,相关性采用Pearson直线相关分析.结果 模型组血清ALT、AST升高,Alb下降;HA、PC Ⅲ、Ⅳ C分别为(177.42±61.25)/μg/L、(34.86±7.47)μg/L、(7.32±3.71)μg/L,较对照组明显升高(t=-3.670、-5.661、-3.950,均P<0.01);模型组肝脏组织胶原纤维大量增生,分割肝小叶并包绕成假小叶.模型组外周血中CD4+CD25+Tregs占CD4+T淋巴细胞比例为(7.41±2.15)%,较对照组的(12.88±2.93)%低(t=3.752,P<0.01);模型组脾脏中Tregs为(9.49±1.16)%,较对照组的(13.16±2.36)%亦低(t=2.793,P<0.05).ALT、AST与外周血和脾脏中Tregs水平呈直线负相关(ALT、AST与外周血中Tregs:r分别为-0.727、-0.698,ALT、AST与脾脏中Tregs:r=-0.663、-0.535;均P<0.05);而Alb与外周血和脾脏中Tregs水平无直线相关(r=0.423、0.372,均P>0.05);HA、PCⅢ、ⅣC与外周血和脾脏中Tregs水平呈直线负相关(HA、PCⅢ、ⅣC与外周血中Tregs:r=-0.719、-0.558、-0.792,HA、PCⅢ、ⅣC与脾脏中Tregs:r=-0.424、-0.685、-0.506;均P<0.05).结论 CCl4诱导的大鼠肝纤维化模型CD4+CD25+Tregs的表达降低,其肝纤维化的免疫学发病机制有待进一步研究.
目的 探討CD4+CD25+調節性T淋巴細胞(Tregs)在肝纖維化免疫髮病機製中的作用.方法 26隻大鼠分為對照組(6隻)和模型組(20隻),通過皮下註射四氯化碳(CCl4)建立肝纖維化模型,檢測血清肝功能和纖維化指標,觀察肝髒病理學變化,流式細胞儀測定外週血及脾髒CD4+CD25+Tregs數量的變化.兩組間差異比較採用t檢驗,相關性採用Pearson直線相關分析.結果 模型組血清ALT、AST升高,Alb下降;HA、PC Ⅲ、Ⅳ C分彆為(177.42±61.25)/μg/L、(34.86±7.47)μg/L、(7.32±3.71)μg/L,較對照組明顯升高(t=-3.670、-5.661、-3.950,均P<0.01);模型組肝髒組織膠原纖維大量增生,分割肝小葉併包繞成假小葉.模型組外週血中CD4+CD25+Tregs佔CD4+T淋巴細胞比例為(7.41±2.15)%,較對照組的(12.88±2.93)%低(t=3.752,P<0.01);模型組脾髒中Tregs為(9.49±1.16)%,較對照組的(13.16±2.36)%亦低(t=2.793,P<0.05).ALT、AST與外週血和脾髒中Tregs水平呈直線負相關(ALT、AST與外週血中Tregs:r分彆為-0.727、-0.698,ALT、AST與脾髒中Tregs:r=-0.663、-0.535;均P<0.05);而Alb與外週血和脾髒中Tregs水平無直線相關(r=0.423、0.372,均P>0.05);HA、PCⅢ、ⅣC與外週血和脾髒中Tregs水平呈直線負相關(HA、PCⅢ、ⅣC與外週血中Tregs:r=-0.719、-0.558、-0.792,HA、PCⅢ、ⅣC與脾髒中Tregs:r=-0.424、-0.685、-0.506;均P<0.05).結論 CCl4誘導的大鼠肝纖維化模型CD4+CD25+Tregs的錶達降低,其肝纖維化的免疫學髮病機製有待進一步研究.
목적 탐토CD4+CD25+조절성T림파세포(Tregs)재간섬유화면역발병궤제중적작용.방법 26지대서분위대조조(6지)화모형조(20지),통과피하주사사록화탄(CCl4)건립간섬유화모형,검측혈청간공능화섬유화지표,관찰간장병이학변화,류식세포의측정외주혈급비장CD4+CD25+Tregs수량적변화.량조간차이비교채용t검험,상관성채용Pearson직선상관분석.결과 모형조혈청ALT、AST승고,Alb하강;HA、PC Ⅲ、Ⅳ C분별위(177.42±61.25)/μg/L、(34.86±7.47)μg/L、(7.32±3.71)μg/L,교대조조명현승고(t=-3.670、-5.661、-3.950,균P<0.01);모형조간장조직효원섬유대량증생,분할간소협병포요성가소협.모형조외주혈중CD4+CD25+Tregs점CD4+T림파세포비례위(7.41±2.15)%,교대조조적(12.88±2.93)%저(t=3.752,P<0.01);모형조비장중Tregs위(9.49±1.16)%,교대조조적(13.16±2.36)%역저(t=2.793,P<0.05).ALT、AST여외주혈화비장중Tregs수평정직선부상관(ALT、AST여외주혈중Tregs:r분별위-0.727、-0.698,ALT、AST여비장중Tregs:r=-0.663、-0.535;균P<0.05);이Alb여외주혈화비장중Tregs수평무직선상관(r=0.423、0.372,균P>0.05);HA、PCⅢ、ⅣC여외주혈화비장중Tregs수평정직선부상관(HA、PCⅢ、ⅣC여외주혈중Tregs:r=-0.719、-0.558、-0.792,HA、PCⅢ、ⅣC여비장중Tregs:r=-0.424、-0.685、-0.506;균P<0.05).결론 CCl4유도적대서간섬유화모형CD4+CD25+Tregs적표체강저,기간섬유화적면역학발병궤제유대진일보연구.
Objective To investigate the role of CD4+ CD25+ regulatory T lymphocyte(Tregs)in the immunological pathogenesis of liver fibrosis.Methods Twenty-six rats were divided into two groups:control group(6 rats)and model group(twenty rats).The rat model of liver fibrosis was induced bv subcutaneous injection of carbon tetrachloride(CCl4).The serums were collected for detection of hepatic function and fibrosis parameters.Hepatic tissue samples were used to observe the histopathological changes.The flow cytometry was used to detect the proportions of Tregs in both peripheral blood and spleen.The data were evaluated by t-test.The relationship between two variables was analyzed using Pearson linear correlation.Results The levels of serum alanine aminotransferase (ALT)and aspartate aminotransferase (AST) were significantly increased,but the level of serum albumin (Alb) was obviously decreased.The concentrations of serum hyaluronic acid (HA),procollagen type Ⅲ(PCⅢ),collagen type Ⅳ(CⅣ)of the model group increased to(177.42±61.25)μg/L,(34.86±7.47)μg/L and(7.32±3.71)μg/L,respectively,which were higher than those in the control group(t=-3.670,-5.661,-3.950,respectively;all P<0.01).In model group,hepatic lobules were full of collagen fibers and the hepatic pseudolobule formation was observed.The proportion of peripheral blood Tregs in CD4+ T lymphocyte in liver fibrosis model was(7.41±2.15)%,which was significantly lower than that in control group(12.88±2.93)%(t=3.752,P<0.01).Furthermore,the frequency of Tregs in spleen of the model group was(9.49±1.16)%,which was also significantly lower than that in control group(13.16±2.36)%(t=2.793,P<0.05).In addition,the levels of serum ALT,AST and fibrosis parameters were inverselv correlated with the frequency of Tregs in spleens and peripheral blood(ALT and Tregs in blood:r=-0.727,AST and Tregs in blood:r=-0.698,ALT and Tregs in spleen:r=-0.663,AST and Tregs in spleen:r=-0.535,HA and Tregs in blood:r=-0.719,PCⅢ and Tregs in blood:r=-0.558,CⅣ and Tregs in blood:r=-0.792,HA and Tregs in spleen:,r=-0.424,PCⅢ and Tregs in spleen:r=-0.685,CⅣ and Tregs in spleen:r=-0.506;all P<0.05).However,the linear correlations between serum Alb and Tregs in spleens and peripheral bloods were not observed(r=0.423,0.372,respectively,both P>0.05).Conclusion These findings suggest that the reduction of CD4+ CD25+ Tregs probably play an important role in the immunological pathogenesis of liver fibrosis.
