中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2008年
12期
1240-1242
,共3页
周诚%吴星%黄维金%蓝海云%辜文洁%祁自柏%梁争论%李河民
週誠%吳星%黃維金%藍海雲%辜文潔%祁自柏%樑爭論%李河民
주성%오성%황유금%람해운%고문길%기자백%량쟁론%리하민
乙型肝炎病毒%核酸检测%献血员筛查
乙型肝炎病毒%覈痠檢測%獻血員篩查
을형간염병독%핵산검측%헌혈원사사
Hepatitis B virus%Nucleic acid testing%Blood donors screening
目的 了解HBV/HCV/HIV联合核酸检测的临床应用.方法 使用Abbott Architecti2000化学发光检测盒对534份血浆样品进行血清学检测,Roche COBAS AmpliPrep/COBAS TaqManHBV Test试剂定量检测分别与2种联合核酸检测结果 进行比较分析.结果 81份HBsAg、HBeAg、抗-HBc 3项均阳性样品联合核酸检测均为HBV阳性,200份HBsAg阴性的样品中联合核酸检测试剂分别有11、19份检测为HBV阳性.HBV DNA定量检测>500 IU/ml的193份样品联合核酸检测试剂阳性符合率分别为96.9%、94.3%,117份样品<500 IU/ml阳性符合率分别为40.2%、45.3%,151份HBV DNA阴性样品联合试剂阴性符合率为99.3%、96.0%.结论 中国人群中存在一定比例低载量HBV携带者,联合核酸试剂作为献血员筛查的补充方法 在提高血液及其制品使用的安全性方面具有一定的临床使用意义.
目的 瞭解HBV/HCV/HIV聯閤覈痠檢測的臨床應用.方法 使用Abbott Architecti2000化學髮光檢測盒對534份血漿樣品進行血清學檢測,Roche COBAS AmpliPrep/COBAS TaqManHBV Test試劑定量檢測分彆與2種聯閤覈痠檢測結果 進行比較分析.結果 81份HBsAg、HBeAg、抗-HBc 3項均暘性樣品聯閤覈痠檢測均為HBV暘性,200份HBsAg陰性的樣品中聯閤覈痠檢測試劑分彆有11、19份檢測為HBV暘性.HBV DNA定量檢測>500 IU/ml的193份樣品聯閤覈痠檢測試劑暘性符閤率分彆為96.9%、94.3%,117份樣品<500 IU/ml暘性符閤率分彆為40.2%、45.3%,151份HBV DNA陰性樣品聯閤試劑陰性符閤率為99.3%、96.0%.結論 中國人群中存在一定比例低載量HBV攜帶者,聯閤覈痠試劑作為獻血員篩查的補充方法 在提高血液及其製品使用的安全性方麵具有一定的臨床使用意義.
목적 료해HBV/HCV/HIV연합핵산검측적림상응용.방법 사용Abbott Architecti2000화학발광검측합대534빈혈장양품진행혈청학검측,Roche COBAS AmpliPrep/COBAS TaqManHBV Test시제정량검측분별여2충연합핵산검측결과 진행비교분석.결과 81빈HBsAg、HBeAg、항-HBc 3항균양성양품연합핵산검측균위HBV양성,200빈HBsAg음성적양품중연합핵산검측시제분별유11、19빈검측위HBV양성.HBV DNA정량검측>500 IU/ml적193빈양품연합핵산검측시제양성부합솔분별위96.9%、94.3%,117빈양품<500 IU/ml양성부합솔분별위40.2%、45.3%,151빈HBV DNA음성양품연합시제음성부합솔위99.3%、96.0%.결론 중국인군중존재일정비례저재량HBV휴대자,연합핵산시제작위헌혈원사사적보충방법 재제고혈액급기제품사용적안전성방면구유일정적림상사용의의.
Objective To evaluate the multiplex nucleic acid testing (NAT) assays for HBV,HCV and HIV in detecting HBV DNA in plasma samples. Methods 534 plasma samples collected form several areas were detected with Abbott Architect i2000 HBsAg, ani-HBs, HBeAg, anti-HBe, anti-HBc and anti-HBc IgM diagnostic kits. HBV DNA levels of those samples were detected with Roche COBAS AmpliPrep/ COBAS TaqMan HBV Test. Two kinds of multiplex NAT assays for HBV, HCV and HIV were used to test HBV DNA of those 534 samples. Results of serology-markers and quantitative HBV DNA levels with results of NAT were compared. Results HBV DNA was positive in all 81 HBsAg, HBeAg and anti-HBc positive samples,detected by both of NAT assays. HBV DNA was positive in 11 and 19 of 200 HBsAg negative samples when detected with the two kinds of NAT assays separately. Compared with the quantitative results detected by Roche COBAS AmpliPrep/COBAS TaqMan HBV Test, the HBV DNA positive rates were 96.9% and 94.3% in 193 samples of HBV DNA levels over 500 IU/ml while 40.2% and 45.3% in 117 samples of HBV DNA levels below 500 IU/ml while 99.3% and 96.0% in 151 samples of DNA negative HBV. Conclusion There are some occult low level HBV DNA carriers with HBsAg negative results in China. NAT assays for HBV, HCV and HIV may be useful to improve the transfusion safety.
