CAJ | 학술논문

目的探讨甘草酸苷对斑秃患者外周血单一核细胞(PBMC)γ干扰素(IFN-γ)、肿瘤坏死因子β(TNF-β)表达的调节作用.方法轻度斑秃患者18例、重症斑秃患者24例及正常人20例为检测对象,用密度梯度离心法常规分离PBMC,用逆转录-聚合酶链反应检测PBMC经植物血凝素和复方甘草酸苷共同刺激后IFN-γ和TNF-β的表达水平.结果重症斑秃组患者IFN-γ及TNF-β水平均明显高于轻度斑秃组及正常人对照组(P值均＜ 0.05),轻度斑秃组高于正常人对照组(P值均＜0.05).甘草酸苷与植物血凝素共同刺激斑秃患者PBMC后可下调IFN--γ及TNF-β表达水平(P值均＜0.05).结论甘草酸苷可抑制斑秃患者Th1型细胞因子表达,逆转Th1型反应.
목적 탐토감초산감대반독환자외주혈단일핵세포(PBMC)γ간우소(IFN-γ)、종류배사인자β(TNF-β)표체적조절작용.방법 경도반독환자18례、중증반독환자24례급정상인20례위검측대상,용밀도제도리심법상규분리PBMC,용역전록-취합매련반응검측PBMC경식물혈응소화복방감초산감공동자격후IFN-γ화TNF-β적표체수평.결과 중증반독조환자IFN-γ급TNF-β수평균명현고우경도반독조급정상인대조조(P치균＜ 0.05),경도반독조고우정상인대조조(P치균＜0.05).감초산감여식물혈응소공동자격반독환자PBMC후가하조IFN--γ급TNF-β표체수평(P치균＜0.05).결론 감초산감가억제반독환자Th1형세포인자표체,역전Th1형반응.
Objective To observe the regulatory effect of glycyrrhizin on the expression of IFN-γ and TNF-β in peripheral blood mononuclear cells (PBMCs) of patients with alopecia areata.Methods PBMCs were obtained from 18 patients with mild alopecia areata,24 patients with severe alopecia areata and 20 normal human controls,and cultured with phytohemagglutinin (PHA) or the combination of PHA and glycyrrhizin for 24 hours.Then,reverse transcription (RT)-PCR was conducted to detect the mRNA expression of IFN-γand TNF-β in these cells.Results The mRNA expression levels of IFN-γand TNF-β in PBMCs were significantly higher in patients with severe alopecia areata than in those with mild alopecia areata and normal human controls (all P ＜ 0.05),and higher in patients with mild alopecia areata than in normal human controls (both P ＜ 0.05).A significant decrease was observed in the mRNA expressions of IFN-γ and TNF-β in the PBMCs from patients with alopecia areata after stimulation with the combination of PHA and glycyrrhizin (both P ＜0.05).Conclusion Glycyrrhizin can inhibit the expression of Th1-type cytokines and reverse Th1-type immune response.