草业学报
草業學報
초업학보
PRATACULTURAL SCIENCE
2009年
6期
180-185
,共6页
薛延丰%李慧明%易能%李优琴%石志琦
薛延豐%李慧明%易能%李優琴%石誌琦
설연봉%리혜명%역능%리우금%석지기
藻毒素%白三叶%种子%抗氧化酶
藻毒素%白三葉%種子%抗氧化酶
조독소%백삼협%충자%항양화매
MC-RR%Trifolium repens%seed%antioxidant enzyme
以白三叶种子和幼苗为材料,研究了不同浓度MC-RR对白三叶种子萌发、抗氧化酶活性变化以及MC-RR在白三叶体内积累规律.结果表明,发芽率和发芽势随着处理浓度的增加而降低,在高浓度处理下,与对照相比显著降低;株高随着MC-RR处理浓度增加而显著降低,根长和鲜重在低浓度处理下显著增加,在高浓度处理下显著降低;抗氧化酶活性的变化不尽相同,SOD活性随着处理浓度的增加与对照相比呈先增加后降低而后又增加的趋势,POD活性随着处理浓度的增加呈先增加后降低的趋势,CAT活性的变化趋势不同于SOD和POD;单位鲜重白三叶MC-RR含量随着处理浓度的增加与0.05 mg/L处理下的MC-RR含量相比显著增加,而生物富集系数的变化趋势则与单位鲜重白三叶MC-RR含量相反,随着MC-RR处理浓度的增加而降低.说明随着MC-RR处理浓度的增加,白三叶受到的伤害增大.
以白三葉種子和幼苗為材料,研究瞭不同濃度MC-RR對白三葉種子萌髮、抗氧化酶活性變化以及MC-RR在白三葉體內積纍規律.結果錶明,髮芽率和髮芽勢隨著處理濃度的增加而降低,在高濃度處理下,與對照相比顯著降低;株高隨著MC-RR處理濃度增加而顯著降低,根長和鮮重在低濃度處理下顯著增加,在高濃度處理下顯著降低;抗氧化酶活性的變化不儘相同,SOD活性隨著處理濃度的增加與對照相比呈先增加後降低而後又增加的趨勢,POD活性隨著處理濃度的增加呈先增加後降低的趨勢,CAT活性的變化趨勢不同于SOD和POD;單位鮮重白三葉MC-RR含量隨著處理濃度的增加與0.05 mg/L處理下的MC-RR含量相比顯著增加,而生物富集繫數的變化趨勢則與單位鮮重白三葉MC-RR含量相反,隨著MC-RR處理濃度的增加而降低.說明隨著MC-RR處理濃度的增加,白三葉受到的傷害增大.
이백삼협충자화유묘위재료,연구료불동농도MC-RR대백삼협충자맹발、항양화매활성변화이급MC-RR재백삼협체내적루규률.결과표명,발아솔화발아세수착처리농도적증가이강저,재고농도처리하,여대조상비현저강저;주고수착MC-RR처리농도증가이현저강저,근장화선중재저농도처리하현저증가,재고농도처리하현저강저;항양화매활성적변화불진상동,SOD활성수착처리농도적증가여대조상비정선증가후강저이후우증가적추세,POD활성수착처리농도적증가정선증가후강저적추세,CAT활성적변화추세불동우SOD화POD;단위선중백삼협MC-RR함량수착처리농도적증가여0.05 mg/L처리하적MC-RR함량상비현저증가,이생물부집계수적변화추세칙여단위선중백삼협MC-RR함량상반,수착MC-RR처리농도적증가이강저.설명수착MC-RR처리농도적증가,백삼협수도적상해증대.
The seed and seedlings of Trifolium repens were treated with various concentrations of MC-RR. The effects of MC-RR on the seed germination, the change of antioxidant enzymes and the regulation of MC-RR accumulation in T. repens were studied in this paper. The results showed that the germination percentage and the germinating viability decreased with the increase of MC-RR concentration. When at the higher concentration, the germination percentage and the germinating viability significantly decreased compared with the control. Shoot height markedly inhibited with the rising of MC-RR concentration. Root length and fresh weight significantly increased under the lower concentration, whereas they markedly reduced at the higher concentration. The changes of antioxidant enzymes were different each other. SOD activity indicated the trend of increase-decrease-increase compared with the control. POD activity illustrated that it increased firstly and then decreased. However, the trend of CAT activity was different from SOD and POD. The MC-RR content of T. repens increased with the treating concentration compared with the concentration of 0.05 mg/L, whereas bioconcentration factor (BCF) decreased. All the results suggested that the seed and seedlings of T. repens would be damaged seriously with the increase of MC-RR concentration.