湖北农业科学
湖北農業科學
호북농업과학
2009年
10期
2451-2455
,共5页
农杆菌素MI15%层析%HPLC%质谱
農桿菌素MI15%層析%HPLC%質譜
농간균소MI15%층석%HPLC%질보
Agrocin MI15%chromatography%HPLC%mass-spectrum
放射土壤杆菌(Agrobacterium radiobacter)MI15菌株.属于生物Ⅰ型.在平皿培养时,能够产生农杆菌素MI15,能抑制葡萄根癌土壤杆菌的生长.采用透析和DEAE纤维素柱离子交换层析纯化制备了MI15菌株分泌的农杆菌素MI15.经HPLC分析,此制备物纯度较高,可达到结构分析要求.紫外吸收光谱分析显示,该物质在260nm和280nm处均无洗脱峰,故认为该物质不是核酸、蛋白类似物.经红外吸收和元素分析,初步认定它是一种小分子有机物,质谱测定结果显示,农杆菌素MI15的分子量为368.
放射土壤桿菌(Agrobacterium radiobacter)MI15菌株.屬于生物Ⅰ型.在平皿培養時,能夠產生農桿菌素MI15,能抑製葡萄根癌土壤桿菌的生長.採用透析和DEAE纖維素柱離子交換層析純化製備瞭MI15菌株分泌的農桿菌素MI15.經HPLC分析,此製備物純度較高,可達到結構分析要求.紫外吸收光譜分析顯示,該物質在260nm和280nm處均無洗脫峰,故認為該物質不是覈痠、蛋白類似物.經紅外吸收和元素分析,初步認定它是一種小分子有機物,質譜測定結果顯示,農桿菌素MI15的分子量為368.
방사토양간균(Agrobacterium radiobacter)MI15균주.속우생물Ⅰ형.재평명배양시,능구산생농간균소MI15,능억제포도근암토양간균적생장.채용투석화DEAE섬유소주리자교환층석순화제비료MI15균주분비적농간균소MI15.경HPLC분석,차제비물순도교고,가체도결구분석요구.자외흡수광보분석현시,해물질재260nm화280nm처균무세탈봉,고인위해물질불시핵산、단백유사물.경홍외흡수화원소분석,초보인정타시일충소분자유궤물,질보측정결과현시,농간균소MI15적분자량위368.
The MI15 strain of Agrobacterium radiobacter can produce Agrocin MI15 which inhibits the growth of A grobactium tumefacwns,a pathogen causing grape crown gall. Agrocin MI 15 was purified with dialysis followed by DEAE-cellulose column chromatography. HPLC analysis showed that a pure Agrocin MI 15 was obtained. UV spectrophotometric analysis indicated that the Agrocin MI 15 was not nucleic acid or protein-like substance because Agrocin MI 15 had no absorption spectrum at 260nm and 280nm. IR spectroscopy and element analysis proved Agrocin MI 15 to be a small molecular organic sub-strance. MS analysis indicated that the molecular weight of Agrocin MI 15 was 368.
