CAJ | 학술논문

采用三电极体系的芯片毛细管电泳安培柱端检测器检测具有还原性的联苯胺,由于敌百虫能够催化过硼酸钠氧化联苯胺的反应,且反应后联苯胺浓度的减少量与敌百虫的加入浓度有线性关系,据此建立了间接检测敌百虫的芯片毛细管电泳法.考察了联苯胺的浓度、缓冲液的pH值、进样时间、分离电压、检测电位等对分离效率和检测信号的影响.在优化实验条件下,敌百虫的线性范围为20 ～400 μg/L,相关系数为0.991 3,检出限为10 μg/L.应用该法测定大白菜样品中敌百虫的残留量,结果满意.
채용삼전겁체계적심편모세관전영안배주단검측기검측구유환원성적련분알,유우활백충능구최화과붕산납양화련분알적반응,차반응후련분알농도적감소량여활백충적가입농도유선성관계,거차건립료간접검측활백충적심편모세관전영법.고찰료련분알적농도、완충액적pH치、진양시간、분리전압、검측전위등대분리효솔화검측신호적영향.재우화실험조건하,활백충적선성범위위20 ～400 μg/L,상관계수위0.991 3,검출한위10 μg/L.응용해법측정대백채양품중활백충적잔류량,결과만의.
A novel method was used to detect reduction benzidine by microchip capillary electrophoresis with an end-column amperometric detector at the three-electrodes system.According to the fact that trichlorfon has the capacity to catalyze the oxidation of benzidine in the presence of sodium perborate,a linear relationship between the concentration of trichlorfon and the decreasing concentration of benzidine was obtained.Based on this,a method for the indirect determination of trichlorfon was developed.The effect of experimental factors,such as concentration and pH of buffer solution,injection time,separation voltage and detection potential,on separation efficiency and amperometric response were assessed and optimized.Under the optimized experimental conditions,there is a linear relationship between trichlorfon in the concentration range of 20-400 μg/L and the peak areas of benzidine(r=0.991 3).The limit of detection was 10 μg/L.