CAJ | 학술논문

目的探讨胶质细胞代谢在延髓基本节律性呼吸放电的作用.方法制备12只新生SD大鼠(0～3 d)离体延髓脑片标本,以改良的Kreb's液(MKS)恒温灌流,稳定记录到与之相连的舌下神经的呼吸节律性放电活动(RRDA)后,第一组在灌流液中分别单独给予胶质细胞代谢激动剂L-谷氨酰胺(L-GLN)和拮抗剂L-硫酸蛋氨酸(L-MSO),第二组先后给予L-MSO和L-MSO+L-GLN,观察舌下神经根RRDA变化.结果给予L-MSO后,呼吸周期(RC)和呼气时程(TE)显著延长,吸气时程(TI)和放电积分幅度(IA)降低;给予L-GLN后RC、TE明显缩短,TI、IA无明显变化,且L-MSO的呼吸抑制作用可被L-GLN逆转.结论胶质细胞代谢在哺乳动物基本节律性呼吸的调节中起着重要的作用.
목적 탐토효질세포대사재연수기본절률성호흡방전적작용.방법 제비12지신생SD대서(0～3 d)리체연수뇌편표본,이개량적Kreb's액(MKS)항온관류,은정기록도여지상련적설하신경적호흡절률성방전활동(RRDA)후,제일조재관류액중분별단독급여효질세포대사격동제L-곡안선알(L-GLN)화길항제L-류산단안산(L-MSO),제이조선후급여L-MSO화L-MSO+L-GLN,관찰설하신경근RRDA변화.결과 급여L-MSO후,호흡주기(RC)화호기시정(TE)현저연장,흡기시정(TI)화방전적분폭도(IA)강저;급여L-GLN후RC、TE명현축단,TI、IA무명현변화,차L-MSO적호흡억제작용가피L-GLN역전.결론 효질세포대사재포유동물기본절률성호흡적조절중기착중요적작용.
Objective To explore the role of glial cell metabolism in the generation and regulation of central respiratory rhythm. Methods The medulla obiongata slices (600-700 μm) containing the medial region of the nucleus retrofacialis (mNRF) with the hypogiossal nerve rootlets retained from 12 neonatal (0-3 days) Sprague-Dawley rats were prepared and perfused with modified Kreb's solution (MKS). Upon recording of respiratory rhythmical discharge activity (RRDA) of the rootlets of the hypoglossal nerve, the brain slices were treated with glial cell metabolism antagonist L-methionine sulfoximine (L-MSO, 50 μmol/L) for 20 min followed by application of glial cell metabolism agonist L-glutamine (L-GLN, 30 μmol/L) for 20 min, or with L-MSO for 20 min with additional L-GLN for 20 min. The changes in the RRDA of the rootlets of the hypoglossal nerve in response to the treatments were recorded. Results L-MSO prolonged the respiratory cycle (RC) and expiratory time (TE), and reduced the integral amplitude (IA) and the inspiratory time (TI) in the brain slices. L-GLN induced a significant decrease in RC and TE, but IA and TI showed no obvious variations. The effect of L-MSO on the respiratory rhythm was reversed by the application of L-GLN. Conclusion Glial cell metabolism may play an important role in the modulation of RRDA in neonatal rat brainstem.