CAJ | 학술논문

采用针对16SrRNA和18SrRNA基因的引物,对武汉东湖不同营养水平湖区的浮游生物宏基因组进行凝胶电泳分析(PCR-DGGE)的指纹分析,并探讨宏基因组与环境重金属的关系.结粜表明,PCR-DGGE分析共获得82条谱带,其多态率为82.9%,特有谱带占31.7%.Ⅰ-Ⅴ站点的谱带分别为36,34,44,50,56条.5个站点浮游生物群落PCR-DGGE指纹结构的聚类分析(UPGMA)分成两大类:Ⅰ、Ⅱ、Ⅲ站为一类,Ⅳ、Ⅴ站为一类,这与重金属(铅、铬、汞、镉、砷、铜)浓度对各站点的聚类分枝关系相一致.相关分析显示,变性梯度凝胶电泳(DGGE)谱带所代表的分类单元数(OTUs)与镉、铬的浓度存在较明显的相关性,其相关系数分别为-0.942(P<0.05)和-0.928(P<0.05).
채용침대16SrRNA화18SrRNA기인적인물,대무한동호불동영양수평호구적부유생물굉기인조진행응효전영분석(PCR-DGGE)적지문분석,병탐토굉기인조여배경중금속적관계.결조표명,PCR-DGGE분석공획득82조보대,기다태솔위82.9%,특유보대점31.7%.Ⅰ-Ⅴ참점적보대분별위36,34,44,50,56조.5개참점부유생물군락PCR-DGGE지문결구적취류분석(UPGMA)분성량대류:Ⅰ、Ⅱ、Ⅲ참위일류,Ⅳ、Ⅴ참위일류,저여중금속(연、락、홍、력、신、동)농도대각참점적취류분지관계상일치.상관분석현시,변성제도응효전영(DGGE)보대소대표적분류단원수(OTUs)여력、락적농도존재교명현적상관성,기상관계수분별위-0.942(P<0.05)화-0.928(P<0.05).
Metagenome of plankton community in Lake Donghu was investigated by PCR-DGGE fingerprinting of the 16S rRNA and 18S rRNA genes, respectively. Then the relationships between the DGGE fingerprints of the plankton community and heavy metals were explored. A total of 82 DGGE bands were detected, of which 82.9% were polymorphic bands and 31.7% were unique bands. The bands detected at station I～V were 36,34,44,50 and 56, respectively. The five investigated stations were classified into two groups using UPGMA clustering based on the DGGE fingerprints of plankton community. Station I and II were found to initially group together into one cluster, which was later joined by station III. Station IV and V made up the second group. These group divisions were consistent with the UPGMA clustering based on the concentrations of the heavy metals (Pb, Cr, Hg, Cd, As, Cu). The correlation analysis indicated that the operational taxonomic units (OTUs, one band-one OTU in principle) were correlated with the heavy metals of Cd (R= -0.942, P < 0.05) and Cr (R = -0.928, P < 0.05).