CAJ | 학술논문

目的:比较3种不同浓度(3.6%,2.4%和1.2%)七氟醚预处理对内毒素性急性肺损伤大鼠肺组织的影响.方法:健康雄性SD大鼠36只,随机分为6组(n=6):对照组(A组)、单纯七氟醚吸入组(B组)、3.6%七氟醚预处理组(C组)、2.4%七氟醚预处理组(D组)、1.2%七氟醚预处理组(E组)、内毒素组(F组).分别在给药后(LPS或生理盐水)6 h 处死大鼠, 观察肺组织病理学结果,测定肺组织湿干比(W/ D)、髓过氧化物酶(MPO)活性及肺组织ICAM-1mRNA的表达.结果:与对照组比较,F组和不同浓度七氟醚预处理组肺组织病理损伤加重,肺W/D、MPO活性、ICAM-1mRNA表达升高(P＜0.05).与F组比较,C组肺组织病理损伤减轻,MPO活性和ICAM 1mRNA表达降低(P＜0.05),但肺W/D无明显降低(P＞0.05);D组肺组织病理损伤减轻,肺W/D、MPO活性、ICAM-1mRNA表达均降低(P＜0.05),F组肺组织MPO活性降低(P＜0.05),但肺W/D和ICAM-1mRNA表达无明显降低(P＞0.05).结论:2.4%七氟醚预处理可以减轻内毒素所致急性肺损伤,作用机制可能与其降低肺组织ICAM-1mRNA的表达上调,从而减少肺内中性粒细胞的浸润相关.
목적:비교3충불동농도(3.6%,2.4%화1.2%)칠불미예처리대내독소성급성폐손상대서폐조직적영향.방법:건강웅성SD대서36지,수궤분위6조(n=6):대조조(A조)、단순칠불미흡입조(B조)、3.6%칠불미예처리조(C조)、2.4%칠불미예처리조(D조)、1.2%칠불미예처리조(E조)、내독소조(F조).분별재급약후(LPS혹생리염수)6 h 처사대서, 관찰폐조직병이학결과,측정폐조직습간비(W/ D)、수과양화물매(MPO)활성급폐조직ICAM-1mRNA적표체.결과:여대조조비교,F조화불동농도칠불미예처리조폐조직병리손상가중,폐W/D、MPO활성、ICAM-1mRNA표체승고(P＜0.05).여F조비교,C조폐조직병리손상감경,MPO활성화ICAM 1mRNA표체강저(P＜0.05),단폐W/D무명현강저(P＞0.05);D조폐조직병리손상감경,폐W/D、MPO활성、ICAM-1mRNA표체균강저(P＜0.05),F조폐조직MPO활성강저(P＜0.05),단폐W/D화ICAM-1mRNA표체무명현강저(P＞0.05).결론:2.4%칠불미예처리가이감경내독소소치급성폐손상,작용궤제가능여기강저폐조직ICAM-1mRNA적표체상조,종이감소폐내중성립세포적침윤상관.
Objective To investigate the effect of 3 concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats. Methods Thirty-six male SD rats were randomly divided into 6 groups:a control group(Group A), a sevoflurane group(Group B), a 3.6% sevoflurane pretreatment group(Group C), a 2.4% Sevoflurane pretreatment group(Group D), a 1.2% sevo sevoflurane pretreatment group(Group E), and lipopolysaccharide (Group F).The rats were killed 6 h after lipopolysaccharide (LPS) or saline injection.Histological examinations of the lung tissues were performed with light microscope. Lung wet/dry weight (W/D) ratio, myeloperoxidase (MPO) activity, and intercellular adhesion molecule 1 (ICAM-1) mRNA expression were assayed. Results Introvenous LPS significantly aggravated lung tissue damage,increased lung W/D ratio, MPO activity, and lung ICAM-1 mRNA expression compared with the control group(P＜0.05). Precondition with 2.4% sevoflurane significantly attenuated the above mentioned changes induced by LPS (P＜0.05). The 3.6% LPS (Group C) significantly attenuated lung tissue damage and decreased MPO activity,lung ICAM-1 mRNA expression compared with the Group F (P＜0.05),but no significant change in lung W/D ratio was seen (P＞0.05). MPO activity was significantly decreased in Group E (P＜0.05), but lung W/D ratio and lung ICAM- 1 mRNA expression had no significant changes (P＞0.05).Conclusion Precondition with 2.4% sevoflurane can reduce LPS induced lung injury in rats. Decreased expression of ICAM-1 and less accumulation of neutrophils were participated in its mechanism.