CAJ | 학술논문

目的观察神经生长因子(NGF)体外对精子活力的影响,确定NGF的最佳量效和时效关系,探索NGF体外对精子活力的影响以及和男性不育的关系.方法分别用0.1、1、10 μmol/L的NGF对精子活力进行研究,以探讨最佳浓度.将10 μmol/L NGF加入精液中在1 h内每隔10 min用CASA检测精子的活力变化.结果 NGF可显著增加精子的平均速率、直线速率、曲线速率和线性系数,差异有统计学意义(P<0.01);增加鞭打频率差异有统计学意义(P<0.05).1 μmol/L的NGF和0.1 μmol/L的NGF对精子活力的促进作用相比有统计学差异,10 μmol/L浓度组和1 μmol/L浓度组差异没有统计学意义.NGF反应30 min后对精子活力的影响最大,一直保持到1 h以后.结论外源性给予NGF可显著增加健康人和不育症患者a级运动精子比例,促进精子活力.
목적 관찰신경생장인자(NGF)체외대정자활력적영향,학정NGF적최가량효화시효관계,탐색NGF체외대정자활력적영향이급화남성불육적관계.방법 분별용0.1、1、10 μmol/L적NGF대정자활력진행연구,이탐토최가농도.장10 μmol/L NGF가입정액중재1 h내매격10 min용CASA검측정자적활력변화.결과 NGF가현저증가정자적평균속솔、직선속솔、곡선속솔화선성계수,차이유통계학의의(P<0.01);증가편타빈솔차이유통계학의의(P<0.05).1 μmol/L적NGF화0.1 μmol/L적NGF대정자활력적촉진작용상비유통계학차이,10 μmol/L농도조화1 μmol/L농도조차이몰유통계학의의.NGF반응30 min후대정자활력적영향최대,일직보지도1 h이후.결론 외원성급여NGF가현저증가건강인화불육증환자a급운동정자비례,촉진정자활력.
Objective Motility is an important physiological characteristic of a mature sperm.Nerve growth factor(NGF) is a protein essential for the development,maintenance and survival of the peripheral and central nervous systems.NGF and its receptors TrkA and p75 are widely expressed in the testis,accessory reproductive organ,and the epididymal sperms.In the present study,we investigated the role of NGF on human sperm motility.Methods Use 0.1,1 and 10 μmol/L concentrations of NGF,on sperm motility study to investigate the optimal concentration.Use CASA to detect Sperm motility changes every 10 minutes in an hour after 10 μmol/L NGF was added to the semen.Results The parameters of sperm motility increased after NGF incubation had significant difference, in particular,VAP,VSL,VCL,BCF and LIN mean were significantly increased more than 32%.MAD,STR,ALH and WOB mean had no notable difference.Furthermore,NGF promotes the sperm motility in a time- and dose- dependent manner.In addition,the enhancement of NGF on sperm motility was more stronger than those of sperm culture medium.Conclusion Our findings suggest that NGF plays a promoted role in human sperm motility.