CAJ | 학술논문

目的了解宫内乙型肝炎病毒(HBV)感染儿童干扰素-γ(IFN-γ)CA重复序列基因多态性与宫内HBV感染易感性的关系,探讨宫内HBV感染的遗传易感因素.方法母亲为HBV携带者,新生儿出生后按程序使用乙型肝炎疫苗或乙型肝炎疫苗联合乙型肝炎免疫球蛋白进行免疫.出生时外周静脉血HBsAg和(或)HBV DNA阳性并持续阳性6个月以上者为宫内HBV感染者(Ⅰ组).出生时及以后随访中未曾出现过HBsAg或HBVDNA阳性,1岁时抗-HBs达保护滴度以上者为正常免疫儿童(Ⅱ组).选取Ⅰ组42例,Ⅱ组68例,用毛细管电泳法对IFN-γ CA重复序列基因多态性进行测定.结果在42例宫内HBV感染组儿童中IFN-γ CA重复序列基因多态性(CA12)+/(CA12)+减少,42例中仅有5例,占11.90%,(CA12)+/(CA12)-占26.19%,(CA12)-/(CA12)-占61.91%;正常免疫儿童IFN-γ(CA12)+/(CA12)+占26.47%,(CA12)+/(CA12)-占33.82%,(CA12)-/(CA12)-占39.71%.宫内HBV感染组与正常免疫组比较趋势(P=0.0176),宫内HBV感染儿童(CA12)+/(CA12)+较正常免疫儿童减少.宫内HBV感染儿童IFN-γ CA重复12次的频率为25%,比正常免疫儿童IFN-γ CA重复12次的频率43.38%明显降低,χ2=7.548,P=0.0060,差异有统计学意义.结论提示IFN-γ重复序列基因多态性可能在决定个体宫内HBV感染遗传易感性方面有一定意义.
목적료해궁내을형간염병독(HBV)감염인동간우소-γ(IFN-γ)CA중복서렬기인다태성여궁내HBV감염역감성적관계,탐토궁내HBV감염적유전역감인소.방법모친위HBV휴대자,신생인출생후안정서사용을형간염역묘혹을형간염역묘연합을형간염면역구단백진행면역.출생시외주정맥혈HBsAg화(혹)HBV DNA양성병지속양성6개월이상자위궁내HBV감염자(Ⅰ조).출생시급이후수방중미증출현과HBsAg혹HBVDNA양성,1세시항-HBs체보호적도이상자위정상면역인동(Ⅱ조).선취Ⅰ조42례,Ⅱ조68례,용모세관전영법대IFN-γ CA중복서렬기인다태성진행측정.결과재42례궁내HBV감염조인동중IFN-γ CA중복서렬기인다태성(CA12)+/(CA12)+감소,42례중부유5례,점11.90%,(CA12)+/(CA12)-점26.19%,(CA12)-/(CA12)-점61.91%;정상면역인동IFN-γ(CA12)+/(CA12)+점26.47%,(CA12)+/(CA12)-점33.82%,(CA12)-/(CA12)-점39.71%.궁내HBV감염조여정상면역조비교추세(P=0.0176),궁내HBV감염인동(CA12)+/(CA12)+교정상면역인동감소.궁내HBV감염인동IFN-γ CA중복12차적빈솔위25%,비정상면역인동IFN-γ CA중복12차적빈솔43.38%명현강저,χ2=7.548,P=0.0060,차이유통계학의의.결론제시IFN-γ중복서렬기인다태성가능재결정개체궁내HBV감염유전역감성방면유일정의의.