中华病理学杂志
中華病理學雜誌
중화병이학잡지
Chinese Journal of Pathology
2008年
7期
454-457
,共4页
王东林%王燏婵%LU Peng%鄂群%施公胜
王東林%王燏嬋%LU Peng%鄂群%施公勝
왕동림%왕율선%LU Peng%악군%시공성
神经胶质瘤%周期素依赖激酶抑制剂p27%出核因子
神經膠質瘤%週期素依賴激酶抑製劑p27%齣覈因子
신경효질류%주기소의뢰격매억제제p27%출핵인자
Glioma%C yelin-dependent kinase inhibitor p27%Nuclear export factor
目的 探讨出核因子CRM1、p27 10位丝氨酸(Ser10)磷酸化及p27蛋白在胶质瘤中的表达、相互关系及意义.方法 免疫组织化学SP法检测70例胶质瘤和10例非肿瘤对照脑组织标本中CRM1、p27Ser10磷酸化形式及p27蛋白的表达,Western blot检测6例新鲜胶质瘤标本中相应蛋白的表达.结果 CRM1及p27Ser10磷酸化形式在对照脑组织中表达不明显,在低级别胶质瘤中表达较少,在高级别胶质瘤中表达较多,两两比较差异均有统计学意义(P<0.01).p27在对照脑组织中表达明显,其表达水平随肿瘤级别增高而降低,差异有统计学意义(P<0.01).Western blot结果显示CRM1、p27Ser10磷酸化形式在胶质瘤中的表达水平与肿瘤细胞的恶性程度相关.相关分析显示:胶质瘤中CRM1蛋白表达与p27蛋白表达呈负相关(r=0.727,P<0.01),与p27Ser10磷酸化形式呈正相关(rs=0.954,P<0.01),与增殖指标Ki-67表达呈正相关(rs=0.799,P<0.01);p27Ser10磷酸化形式与p27蛋白表达呈负相关(rs=-0.744,P<0.01),与Ki-67表达呈正相关(rs=0.785,P<0.01).结论 在胶质瘤中高表达的CRM1可能通过识别并结合高表达的p27Ser10磷酸化形式,促进p27的出核降解,使p27表达降低,失去对细胞周期的调控,从而促进胶质瘤的恶性进展和增殖.
目的 探討齣覈因子CRM1、p27 10位絲氨痠(Ser10)燐痠化及p27蛋白在膠質瘤中的錶達、相互關繫及意義.方法 免疫組織化學SP法檢測70例膠質瘤和10例非腫瘤對照腦組織標本中CRM1、p27Ser10燐痠化形式及p27蛋白的錶達,Western blot檢測6例新鮮膠質瘤標本中相應蛋白的錶達.結果 CRM1及p27Ser10燐痠化形式在對照腦組織中錶達不明顯,在低級彆膠質瘤中錶達較少,在高級彆膠質瘤中錶達較多,兩兩比較差異均有統計學意義(P<0.01).p27在對照腦組織中錶達明顯,其錶達水平隨腫瘤級彆增高而降低,差異有統計學意義(P<0.01).Western blot結果顯示CRM1、p27Ser10燐痠化形式在膠質瘤中的錶達水平與腫瘤細胞的噁性程度相關.相關分析顯示:膠質瘤中CRM1蛋白錶達與p27蛋白錶達呈負相關(r=0.727,P<0.01),與p27Ser10燐痠化形式呈正相關(rs=0.954,P<0.01),與增殖指標Ki-67錶達呈正相關(rs=0.799,P<0.01);p27Ser10燐痠化形式與p27蛋白錶達呈負相關(rs=-0.744,P<0.01),與Ki-67錶達呈正相關(rs=0.785,P<0.01).結論 在膠質瘤中高錶達的CRM1可能通過識彆併結閤高錶達的p27Ser10燐痠化形式,促進p27的齣覈降解,使p27錶達降低,失去對細胞週期的調控,從而促進膠質瘤的噁性進展和增殖.
목적 탐토출핵인자CRM1、p27 10위사안산(Ser10)린산화급p27단백재효질류중적표체、상호관계급의의.방법 면역조직화학SP법검측70례효질류화10례비종류대조뇌조직표본중CRM1、p27Ser10린산화형식급p27단백적표체,Western blot검측6례신선효질류표본중상응단백적표체.결과 CRM1급p27Ser10린산화형식재대조뇌조직중표체불명현,재저급별효질류중표체교소,재고급별효질류중표체교다,량량비교차이균유통계학의의(P<0.01).p27재대조뇌조직중표체명현,기표체수평수종류급별증고이강저,차이유통계학의의(P<0.01).Western blot결과현시CRM1、p27Ser10린산화형식재효질류중적표체수평여종류세포적악성정도상관.상관분석현시:효질류중CRM1단백표체여p27단백표체정부상관(r=0.727,P<0.01),여p27Ser10린산화형식정정상관(rs=0.954,P<0.01),여증식지표Ki-67표체정정상관(rs=0.799,P<0.01);p27Ser10린산화형식여p27단백표체정부상관(rs=-0.744,P<0.01),여Ki-67표체정정상관(rs=0.785,P<0.01).결론 재효질류중고표체적CRM1가능통과식별병결합고표체적p27Ser10린산화형식,촉진p27적출핵강해,사p27표체강저,실거대세포주기적조공,종이촉진효질류적악성진전화증식.
Objective To investigate the expression of nuclear export factor CRM1, Ser10-phosphorylated p27 and p27 in human gliomas. Methods The expression of CRM1, Ser10-phosphorylatedp27 and p27 were investigated in 70 cases of human gliomas and 10 specimens of the normal brain tissue byimmunohistochemical technique and Western blot. Results There were significant differences on theexpression levels of CRM1, Ser10-phosphorylated p27 and p27 among normal brain tissue, gliomas of gradesⅡ and gliomas of grades Ⅲ plus Ⅳ (P < 0. 01). The expression of CRM1 in gliomas was inverselycorrelated with the expression of p27 (rs =-0. 727, P < 0. 01) and positively correlated with the expressionof Ser10-phosphorylated p27 (rs = 0. 954, P < 0. 01) and Ki-67 (rs = 0. 799, P < 0. 01). Moreover, theexpression of Ser10-phosphorylated p27 was inversely correlated with p27 (rs= - 0. 744, P < 0. 01) andpositively correlated with Ki-67 (r, = 0. 785, P < 0. 01). Conclusions CRM1, through recognizing andbinding with Ser10-phosphorylated p27, may promote moving of p27CRM1 from its original locating sites;act as a critical signaling component in the proliferative process of glioma cells and then, plays an importantrole in the development of gliomas.
