CAJ | 학술논문

目的评价脊髓背角环氧化酶-1(COX-1)和COX-2在p38丝裂原活化蛋白激酶(p38MAPK)诱发大鼠切口痛中的作用.方法雄性SD大鼠,体重250～300 g,选择鞘内置管成功的大鼠112只,随机分为4组(n=28):假手术组(S组)、切口痛组(IP组)、二甲基亚砜组(DMSO组)和p38MAPK特异性抑制剂SB203580组(SB203580组).S组鞘内注射0.9%生理盐水20 μl后,吸入异氟烷(呼气末浓度1.4%)min,不做手术;IP组术前10 min鞘内注射0.9%生理盐水20 μl;DMSO组和SB203580组术前10 min分别鞘内注射2%DMSO(SB203580的溶媒)10 μl和SB203580 30 μg(10 μl),然后用0.9%生理盐水10 μl冲洗导管.于鞘内置管后5 d,制备大鼠左后足切口痛模型.各组于术前1 h、术后2、3、6 h和1、2、3、5 d时,测定机械缩足反射阈值(MWT)和热刺激缩足反射潜伏期(TWL).各组于术后2、3、6 h和1、2、3、5 d痛阈测定结束后各处死4只大鼠,采用Western blotting法测定脊髓背角COX-1和COX-2的表达水平.结果与S组比较,IP组和DMSO组术后2 h～2 d时MWT降低,术后2 h～3 d时TWL缩短,IP组、DMSO组和SB203580组术后3 h～3 d脊髓背角COX-1表达上调(P<0.05);与IP组和DMSO组比较,SB203580组术后2 h～1 d时MWT升高,术后2 h～2 d时TWL延长,术后6 h～2 d脊髓背角COX-1表达下调(P<0.05);4组术后各时点脊髓背角COX-2表达差异无统计学意义(P>0.05).结论 p38MAPK诱发大鼠切口痛与脊髓背角COX-1有关,与COX-2无关.
목적 평개척수배각배양화매-1(COX-1)화COX-2재p38사렬원활화단백격매(p38MAPK)유발대서절구통중적작용.방법 웅성SD대서,체중250～300 g,선택초내치관성공적대서112지,수궤분위4조(n=28):가수술조(S조)、절구통조(IP조)、이갑기아풍조(DMSO조)화p38MAPK특이성억제제SB203580조(SB203580조).S조초내주사0.9%생리염수20 μl후,흡입이불완(호기말농도1.4%)min,불주수술;IP조술전10 min초내주사0.9%생리염수20 μl;DMSO조화SB203580조술전10 min분별초내주사2%DMSO(SB203580적용매)10 μl화SB203580 30 μg(10 μl),연후용0.9%생리염수10 μl충세도관.우초내치관후5 d,제비대서좌후족절구통모형.각조우술전1 h、술후2、3、6 h화1、2、3、5 d시,측정궤계축족반사역치(MWT)화열자격축족반사잠복기(TWL).각조우술후2、3、6 h화1、2、3、5 d통역측정결속후각처사4지대서,채용Western blotting법측정척수배각COX-1화COX-2적표체수평.결과 여S조비교,IP조화DMSO조술후2 h～2 d시MWT강저,술후2 h～3 d시TWL축단,IP조、DMSO조화SB203580조술후3 h～3 d척수배각COX-1표체상조(P<0.05);여IP조화DMSO조비교,SB203580조술후2 h～1 d시MWT승고,술후2 h～2 d시TWL연장,술후6 h～2 d척수배각COX-1표체하조(P<0.05);4조술후각시점척수배각COX-2표체차이무통계학의의(P>0.05).결론 p38MAPK유발대서절구통여척수배각COX-1유관,여COX-2무관.
Objective To evaluate the role of COX-1 and COX-2 in the spinal dorsal horn in incisional pain induced by p38MAPK in rats.Methods Male SD rats weighing 250-300 g were used in this study.The animals were anesthetized with intraperitoneal pentobarbital 40 mg/kg.A PE-10 catheter was inserted into the subarachnoid space with the tip positioned at the lumbar enlargement according to the method described by Yaksh et al.One hundred and twelve SD rats in which IT catheter was successfully placed without any complication were randomly divided into 4 groups with 28 animals in each group: group Ⅰ sham operation (S) ; group Ⅱ incisional pain (IP) : group Ⅲ IT SB203580 + IP and group Ⅳ IT DMSO (the solvent) + IP.An 1 cm long incision was made in the plantar surface according to the method described by Brennan et al.The animals were killed at 2,3,6 h,1,2,3,5 d after operation ( n = 4,at each time point).The L4-5 segment of the spinal cord was removed for determiuation of expression of COX-1 and COX-2 in the spinal dorsal horn (by Western blot).Results Incisional pain significantly increased the expression of COX-1 protein in the spinal dorsal horn at 2,3,6 h and 1,2,3 d after incision in group Ⅱ and Ⅳ as compared with sham operation group.IT SB203580 significantly inhibited the incisional pain induced increase in COX-1 in the spinal dorsal horn in group Ⅲ as compared with group Ⅱ and Ⅳ.There was no significant difference in the expression of COX-2 in the spinal dorsal horn among the 4 groups.Conclusion COX-1 but not COX-2 in the spinal dorsal horn is involved in incisional pain induced by p38MARK.