CAJ | 학술논문

目的探讨小剂量γ射线全身照射后对小鼠中枢神经系统的影响.方法将50只C57小鼠按随机表法分为健康对照组及0.5和1 Gy照射组.用60Co γ射线全身照射,吸收剂量为0.5和1 Gy,吸收剂量率为19.2 cGy/min,连续照射3d,1次/d,照射后24和48 h取小鼠脑组织并匀浆,用高效液相色谱荧光法(HPLC)测定匀浆上清中氨基酸类神经递质(谷氨酸、天冬氨酸、γ-氨基丁酸及甘氨酸)含量.结果与健康对照组相比,0.5Gyγ射线照射后48 h以及1 Gy照射后24和48 h,小鼠脑内谷氨酸和天冬氨酸含量表达增高(t=- 4.080、- 3.935、-4.416、- 3.630、-4.831和- 4.656,P＜0.05)；而0.5Gyγ射线照射后24 h小鼠脑内谷氨酸、天冬氨酸含量无明显改变.各照射组小鼠脑内γ-氨基丁酸和甘氨酸含量与健康对照组比较,差异无统计学意义.结论小剂量γ射线短期全身照射对小鼠中枢神经系统具有轻度的兴奋作用.
목적 탐토소제량γ사선전신조사후대소서중추신경계통적영향.방법 장50지C57소서안수궤표법분위건강대조조급0.5화1 Gy조사조.용60Co γ사선전신조사,흡수제량위0.5화1 Gy,흡수제량솔위19.2 cGy/min,련속조사3d,1차/d,조사후24화48 h취소서뇌조직병균장,용고효액상색보형광법(HPLC)측정균장상청중안기산류신경체질(곡안산、천동안산、γ-안기정산급감안산)함량.결과 여건강대조조상비,0.5Gyγ사선조사후48 h이급1 Gy조사후24화48 h,소서뇌내곡안산화천동안산함량표체증고(t=- 4.080、- 3.935、-4.416、- 3.630、-4.831화- 4.656,P＜0.05)；이0.5Gyγ사선조사후24 h소서뇌내곡안산、천동안산함량무명현개변.각조사조소서뇌내γ-안기정산화감안산함량여건강대조조비교,차이무통계학의의.결론 소제량γ사선단기전신조사대소서중추신경계통구유경도적흥강작용.
Objective To investigate the effect of whole-body irradiation with low-dose γ-rays on the central nervous system of mice.Methods Fifty C57 mice were randomly divided into 3 groups and treated with 0,0.5,1 Gy whole-body irradiation,respectively.24 or 48 h after irradiation,brain tissue of mice was resected and homogenated.The levels of amino acid neurotransmitter,including Glu,Asp,GABA and Gly in brain homogenate were measured by high performance liquid chromatography (HPLC).Results Compared to the brain tissue of untreated mice,the contents of Glu and Asp at 0.5 and 1 Gy (t=-4.080,-3.935,-4.416,-3.630,-4.831, - 4.656,P ＜0.05) in mice brain tissue significantly increased at 24 h at 1 Gy and 48 h.However,the contents of Glu and Asp had no obvious changes in mice brain tissue 24 h after 1 Gy of irradiation. The contents of GABA and Gly had no difference between irradiated groups and untreated control group. Conclusions Short-term whole-body irradiation with low-dose γ-rays induces slight stimulation effect on the central nervous system of mice.