CAJ | 학술논문

目的从形态上证实γ-氨基丁酸(GABA)神经元参与脊髓中间外侧柱胆碱能调节反应,参与热应激时下丘脑对高温调节反应. 方法出生后6周的C57BL小鼠按随机数字表法分为正常对照组和热刺激组,取颈胸端脊髓及下丘脑组织切片,采用免疫组化双标记方法观察小鼠胸脊髓中间外侧柱胆碱能神经元标志物胆碱乙酰转移酶(CHAT)和谷氨酸脱羧酶65(GAD65)的免疫标记情况以及热应激后下丘脑TRPV4及GAD65分别和c-FOS的免疫双标记.结果中间外侧柱交感神经节前神经元存在ChAT和GAD65免疫荧光双标记.热激活后,下丘脑热敏感神经元与GABA能神经元存在双标记.结论 GABA能神经元可能参与中间外侧柱交感神经节前胆碱能神经元调节,以及参与下丘脑对高温的反应.
목적 종형태상증실γ-안기정산(GABA)신경원삼여척수중간외측주담감능조절반응,삼여열응격시하구뇌대고온조절반응. 방법 출생후6주적C57BL소서안수궤수자표법분위정상대조조화열자격조,취경흉단척수급하구뇌조직절편,채용면역조화쌍표기방법관찰소서흉척수중간외측주담감능신경원표지물담감을선전이매(CHAT)화곡안산탈최매65(GAD65)적면역표기정황이급열응격후하구뇌TRPV4급GAD65분별화c-FOS적면역쌍표기.결과 중간외측주교감신경절전신경원존재ChAT화GAD65면역형광쌍표기.열격활후,하구뇌열민감신경원여GABA능신경원존재쌍표기.결론 GABA능신경원가능삼여중간외측주교감신경절전담감능신경원조절,이급삼여하구뇌대고온적반응.
Objective To confirm the regulative effects of GABA neurons on the cholinergic of intermediolateral column of the spinal cord and explore the expressions of GABA neurons in the hypothalamus following thermal stimulation from the point of morphology. Methods C57BL rats born for 6 weeks were randomized into normal control group and thermal stimulation group. The tissue slices from the cervical and thoracic spinal cords and the hypothalamus were got and performed immunohistochemical double labeling to observe the expressions of choline acetyltransferase (ChAT) and glutamic acid decarboxylase-65 (GAD65) in intermediolateral column of the spinal cord and to determine the co-expressions of GABA neurons with c-Fos protein and TRPV with c-Fos protein after heat exposure. Results The GABA maker GAD65 was co-expressed with cholinergic neurons in the intermediolateral column of the spinal cord; so was the GABA neurons with c-Fos protein and TRPV with c-Fos protein in the hypothalamus after heat exposure. Conclusion GABA-ergie neuron may involve in the modulation of acetylcholine releasing in the spinal cord and in the thermoregulation of PO/AH.