中华肾脏病杂志
中華腎髒病雜誌
중화신장병잡지
2011年
10期
735-739
,共5页
李鹏%牛建英%张绮%范伟峰%罗丽红%钱英俊%张丽红%顾勇
李鵬%牛建英%張綺%範偉峰%囉麗紅%錢英俊%張麗紅%顧勇
리붕%우건영%장기%범위봉%라려홍%전영준%장려홍%고용
肾病综合征%水肿%水通道蛋白质类
腎病綜閤徵%水腫%水通道蛋白質類
신병종합정%수종%수통도단백질류
Nephrotic syndrome%Edema%Aquaporins
目的 了解肾组织局部水通道蛋白( AQP)表达与肾病综合征(NS)水肿的关系.方法 NS患者分为水肿组(14例)及无水肿组(8例),以非NS患者为对照组(10例).以免疫组织化学方法检测各组患者肾组织AQP1、2、4表达,分析其与患者水肿程度的关系.结果 NS患者AQP1主要表达在近端肾小管,其中水肿组免疫组化阳性指数为0.0373±0.0110,表达量显著低于非水肿组的0.0510±0.0120和对照组的0.0574±0.0100,差异均有统计学意义;而后两组间差异无统计学意义.AQP1在各组肾小球也有表达,水肿组阳性指数为0.0106±0.0037,显著高于非水肿组的0.0021±0.0013和对照组的0.0020±0.0012,差异均有统计学意义;而后两组间差异无统计学意义.AQP2主要表达在集合管系统,其中水肿组阳性指数为0.0498±0.0081,非水肿组为0.0370±0.0072,均显著高于对照组的0.0255±0.0103,差异均有统计学意义;而水肿组与非水肿组间差异也有统计学意义.AQP4在肾脏皮质集合管未见表达.结论 不同AQP亚型在NS患者肾组织表达有差异.AQP2在NS水肿发生中起着重要作用,而AQP1的表达异常也参与水肿的发生.
目的 瞭解腎組織跼部水通道蛋白( AQP)錶達與腎病綜閤徵(NS)水腫的關繫.方法 NS患者分為水腫組(14例)及無水腫組(8例),以非NS患者為對照組(10例).以免疫組織化學方法檢測各組患者腎組織AQP1、2、4錶達,分析其與患者水腫程度的關繫.結果 NS患者AQP1主要錶達在近耑腎小管,其中水腫組免疫組化暘性指數為0.0373±0.0110,錶達量顯著低于非水腫組的0.0510±0.0120和對照組的0.0574±0.0100,差異均有統計學意義;而後兩組間差異無統計學意義.AQP1在各組腎小毬也有錶達,水腫組暘性指數為0.0106±0.0037,顯著高于非水腫組的0.0021±0.0013和對照組的0.0020±0.0012,差異均有統計學意義;而後兩組間差異無統計學意義.AQP2主要錶達在集閤管繫統,其中水腫組暘性指數為0.0498±0.0081,非水腫組為0.0370±0.0072,均顯著高于對照組的0.0255±0.0103,差異均有統計學意義;而水腫組與非水腫組間差異也有統計學意義.AQP4在腎髒皮質集閤管未見錶達.結論 不同AQP亞型在NS患者腎組織錶達有差異.AQP2在NS水腫髮生中起著重要作用,而AQP1的錶達異常也參與水腫的髮生.
목적 료해신조직국부수통도단백( AQP)표체여신병종합정(NS)수종적관계.방법 NS환자분위수종조(14례)급무수종조(8례),이비NS환자위대조조(10례).이면역조직화학방법검측각조환자신조직AQP1、2、4표체,분석기여환자수종정도적관계.결과 NS환자AQP1주요표체재근단신소관,기중수종조면역조화양성지수위0.0373±0.0110,표체량현저저우비수종조적0.0510±0.0120화대조조적0.0574±0.0100,차이균유통계학의의;이후량조간차이무통계학의의.AQP1재각조신소구야유표체,수종조양성지수위0.0106±0.0037,현저고우비수종조적0.0021±0.0013화대조조적0.0020±0.0012,차이균유통계학의의;이후량조간차이무통계학의의.AQP2주요표체재집합관계통,기중수종조양성지수위0.0498±0.0081,비수종조위0.0370±0.0072,균현저고우대조조적0.0255±0.0103,차이균유통계학의의;이수종조여비수종조간차이야유통계학의의.AQP4재신장피질집합관미견표체.결론 불동AQP아형재NS환자신조직표체유차이.AQP2재NS수종발생중기착중요작용,이AQP1적표체이상야삼여수종적발생.
Objective To elucidate the association between aquaporins (AQPs) expression in kidney tissue and edema of nephrotic syndrome (NS) patients. Methods NS patients were divided into edema group (14 cases) and non-edema group (8 cases).Ten patients without NS were used as control group.Expressions of AQP1,AQP2,and AQP4 in renal tissues of 3 groups were detected by immunohistochemistry with standard techniques and semi-quantitative analysis.Association between AQPs expression and edema was examined. Results The positive index of AQP1 expression in proximal tubules in edema group was 0.0373±0.0110,which was significantly lower as compared to non-edema group (0.0510±0.0120) and control group 0.0574±0.0100),while the difference between non-edema and control groups was not significant.The positive index of AQP1 expression in glomerulus was 0.0106±0.0037 in edema group,which was significantly higher than that in non-edema group (0.0021±0.0013) and control group (0.0020±0.0012),while no significant difference was found between the last two groups.AQP2 mainly localized in the collecting duct system.The positive indexes of AQP2 expression were 0.0498±0.0081,0.0370± 0.0072 and 0.0255±0.0103 in edema group,non-edema group and control group,respectively.The differences were significant among 3 groups.AQP4 expression was not found in the renal cortex and collecting duct system. Conclusions AQPs expression is different in renal tissues of NS patients.AQP2 may play an important role in the edema of NS patients,and AQP1 may involve in the occurrence of edema.