CAJ | 학술논문

目的评价H5N1亚型禽流感病毒免疫血清对实验感染小鼠的紧急预防保护效果.方法给30只小鼠腹腔注射0.2 ml/只禽流感病毒免疫血清,注射后1～19 d内每天采3只小鼠血清测定血凝抑制(HI)抗体效价,测定免疫血清在小鼠体内的消长规律;给10只小鼠腹腔注射1.5 ml/只禽流感病毒免疫血清,检测免疫血清对小鼠的安全性;将70只小鼠按数字表法随机分为7组,分别为正常对照组、病毒对照组以及免疫血清高剂量早期组、高剂量中期组、高剂量晚期组、中剂量组、低剂量组.每组10只,分别在攻毒前8 d,4 d和1 d时腹腔注射血清0.2 ml/只、0.1 ml/只、0.05 ml/只禽流感病毒免疫血清,然后给小鼠滴鼻感染10个半数致死量(LD50)病毒,攻毒后连续观察14 d,计算小鼠存活率和平均存活天数,比较各实验组小鼠肺指数及肺脏病毒感染滴度.结果注射0.2 ml免疫血清小鼠HI抗体效价在1～15 d时为26,在17 d以后开始下降;1.5 ml免疫血清注射小鼠全部存活,没有发病;在攻毒前8 d、4 d和1 d时给小鼠腹腔注射0.2 ml免疫血清,小鼠存活率分别为80%、100%和100%,平均存活天数分别为13.1 d、14.0 d和14.0 d,在攻毒前1 d给小鼠腹腔注射0.1 ml和0.05 ml免疫血清,小鼠存活率分别为100%和50%,平均存活天数分别为14.0 d和11.7 d,各实验组小鼠肺指数(O.0096±0.0033～0.0145±0.0060)均小于病毒对照组(0.0199±0.0025),除低剂量组外,均具有统计学意义(P值0.0022～0.0470,＜0.05),各实验组小鼠肺脏病毒滴度(TCID50)较病毒对照组平均低2个滴度.结论禽流感病毒免疫血清对实验感染小鼠具有较好的紧急预防保护效果,可作为人禽流感紧急预防制剂加以研究开发.
목적 평개H5N1아형금류감병독면역혈청대실험감염소서적긴급예방보호효과.방법 급30지소서복강주사0.2 ml/지금류감병독면역혈청,주사후1～19 d내매천채3지소서혈청측정혈응억제(HI)항체효개,측정면역혈청재소서체내적소장규률;급10지소서복강주사1.5 ml/지금류감병독면역혈청,검측면역혈청대소서적안전성;장70지소서안수자표법수궤분위7조,분별위정상대조조、병독대조조이급면역혈청고제량조기조、고제량중기조、고제량만기조、중제량조、저제량조.매조10지,분별재공독전8 d,4 d화1 d시복강주사혈청0.2 ml/지、0.1 ml/지、0.05 ml/지금류감병독면역혈청,연후급소서적비감염10개반수치사량(LD50)병독,공독후련속관찰14 d,계산소서존활솔화평균존활천수,비교각실험조소서폐지수급폐장병독감염적도.결과주사0.2 ml면역혈청소서HI항체효개재1～15 d시위26,재17 d이후개시하강;1.5 ml면역혈청주사소서전부존활,몰유발병;재공독전8 d、4 d화1 d시급소서복강주사0.2 ml면역혈청,소서존활솔분별위80%、100%화100%,평균존활천수분별위13.1 d、14.0 d화14.0 d,재공독전1 d급소서복강주사0.1 ml화0.05 ml면역혈청,소서존활솔분별위100%화50%,평균존활천수분별위14.0 d화11.7 d,각실험조소서폐지수(O.0096±0.0033～0.0145±0.0060)균소우병독대조조(0.0199±0.0025),제저제량조외,균구유통계학의의(P치0.0022～0.0470,＜0.05),각실험조소서폐장병독적도(TCID50)교병독대조조평균저2개적도.결론금류감병독면역혈청대실험감염소서구유교호적긴급예방보호효과,가작위인금류감긴급예방제제가이연구개발.
Objective To evaluate emergency prophylactic effects of the avian influenza virus immunized serum on experimentally infected mice.Methods Serum HI antibody titers of 30 mice were detected at day 1 to 19 after being inoculated with 0.2 ml immune serum to estimate half life of immune serum.Ten mice clinical symptom was recorded to estimate the serum security after mice injected 1.5 ml immune serum.Seventy mice were randomly divided into 7 groups according to random number table and inoculated with 0.2 ml,0.1 ml and 0.05 ml immune serum respectivdy via intraperitoneal injection on day 8,4 and 1 prior to challenged with 10 LD50 influenza virus intranasaL Mice were observed continually for 14 days to calculate the morbidity,mortality,average survival days and compare the lung index and viral titers in lung.Results Serum HI antibody titers of mice which inoculated with 0.2 ml immune serum maintained 26 in 15 days after injection,but drawdown after day 17,the mice injected 1.5 ml immune serum were all alive and none onset.The survival rate of mice which injected 0.2 ml serum on the day 8,4,1 before challenge was 80% ,100% and 100%,and the average survival period was 13.1 days ,14.0 days and 14.0 days respectively.The survival rate of mice which injected 0.1 ml and 0.05 ml serum on day 1 before challenge was 100% and 50% ,and the average survival days were 14.0 days and 11.7 days respectively.The mice lung index of experimental groups (0.0096 ±0.0033-0.0145±0.0060) was smaller than that of viral control group (0.0199±0.0025) ,with a statistical significance (P value 0.0022 - 0.0470,＜ 0.05).The viral titers in lung were significantly decreased by 2 titer as compared to the viral controls.Conclusion The avian influenza virus immunized serum might contain the emergency prophylactic effects and could be developed as an agent for possible human-avian influenza pandemic.