CAJ | 학술논문

The role of protein kinase B (PKB) in the regulation of early development of fer tilized eggs in mou se and its biological function on the development of mouse 1-cell stage fertili zed eggs were studied.The kinase activity of PKB was detected,and mRNA and prote in level of PKB both in MⅡ oocytes and 1-cell stage fertilized eggs were semi-quantitatively assayed by Western blotting and RT-PCR.10 μmol/L LY294002,the inhibitor of PKB,was microinjected into 1-cell stage fertilized eggs of mouse to observe its effect on the development of mouse 1-cell stage fertilized eggs. The results showed that the activity of PKB increased obviously after the fertil ization,the mRNA and protein level of PKB also increased obviously in 1-cell st age fertilized eggs,while in MⅡ oocytes the mRNA level was not detected.The pr o tein level was lower than that in the 1-cell stage fertilized eggs.After microi njecting LY294002 the development from 1-cell stage to 2-cell stage was inhibi ted obviously.All the results demonstrate that PKB exists in the fertilize d eggs,and the activity,expression and transcription level increase after fertil ization.PKB may play a positive role during the early development of fertilized eggs in mouse from 1-cell to 2-cell stage,but the mechanism is still unknown.T hese experiments are helpful for the further study of PKB on the regulation of e arly development of fertilized eggs.