CAJ | 학술논문

野禽携带的新城疫病毒是新城疫流行传播的重要的潜在传染源.结合本实验室克隆测序的鸽NDV(PB9601)、企鹅NDV(QE01)以及GenBank下载的44株野禽(包括野鸭、鹦鹉、鸽子、天鹅、鸳鸯、雁、火鸡等)的NDV毒株的融合蛋白(Fusion gene,F)基因和血凝素蛋白(Hemagglutinin-neuraminidase gene,HN)基因分别进行了F蛋白裂解位点、基因分型以及F和HN基因氨基酸全长的遗传距离分析.结果表明:野禽感染NDV F基因的基因型以Ⅶ和Ⅵ为主,同时I、II、V和IX多种基因型并存.同一种属、同一地区的毒株间的遗传距离较近,具有明显的种属性和区域性,且绝大多数分离株与经典毒株LaSota、V4、B1、TEX-48等的遗传距离相对较远;对HN基因的遗传进化关系分析得出了相似的结论.
야금휴대적신성역병독시신성역류행전파적중요적잠재전염원.결합본실험실극륭측서적합NDV(PB9601)、기아NDV(QE01)이급GenBank하재적44주야금(포괄야압、앵무、합자、천아、원앙、안、화계등)적NDV독주적융합단백(Fusion gene,F)기인화혈응소단백(Hemagglutinin-neuraminidase gene,HN)기인분별진행료F단백렬해위점、기인분형이급F화HN기인안기산전장적유전거리분석.결과표명:야금감염NDV F기인적기인형이Ⅶ화Ⅵ위주,동시I、II、V화IX다충기인형병존.동일충속、동일지구적독주간적유전거리교근,구유명현적충속성화구역성,차절대다수분리주여경전독주LaSota、V4、B1、TEX-48등적유전거리상대교원;대HN기인적유전진화관계분석득출료상사적결론.
Newcastle disease virus carried by wildfowl is the important reason and potential source which caused Newcastle disease in poultry.Based on 44 wildfowls' (including wild duck, parrot, pigeon, swan ,mandarin duck, wild goose, turkey, etc.) NDV from genbank and the pigeon NDV (PB9601) and the penguin NDV(QE01) cloned and sequenced by our laboratory ,the F gene cleavage site sequence, genotype and the hereditary variation of the deduced amino acids of HN and F gene were analyzed.The results showed the most NDV isolates genotype belonged to Ⅶ and Ⅵ and the others existed different genotypes (I, II, V, IX).Through the hereditary variation analysis of F gene,the isolates had obvious regional and genus traits among the same species or the same regions; most of the isolated strains had farther genetic distance with LaSota, V4, B1, TEX-48.The similar results can be obtained through the genetic evolution of relations of HN genes.