热带作物学报
熱帶作物學報
열대작물학보
CHINESE JOURNAL OF TROPICAL CROPS
2009年
8期
1088-1093
,共6页
洋桔梗切花%1-MCP%ETP%SDS-PAGE%衰老%蛋白质组分变化
洋桔梗切花%1-MCP%ETP%SDS-PAGE%衰老%蛋白質組分變化
양길경절화%1-MCP%ETP%SDS-PAGE%쇠로%단백질조분변화
cut flower of Eustoma grandiflorum%1-MCP%ETP%senescence%SDS-PAGE%changes of protein components
以3个品种洋桔梗为材料.研究洋桔梗切花花瓣衰老过程中.可溶性蛋白质的变化情况.结果表明,1-MCP(1-Methvlcyclopropene.1-甲基环丙烯)在延缓切花衰老,提高观赏品质方面有良好的作用;ETP(乙烯利)促进了蛋白质的分解,缩短了瓶插寿命.切花衰老过程中,蛋白质的组分和含量都发生变化,有些蛋白质始终保持不变.如69.31、49.35、42.82、29.75 ku四条谱带是始终存在;大部分蛋白质分解消失,如98.90、55.66、37.53、27.85、25.63、23.62、19.85、16.74 ku等谱带到瓶插后期基本消失;瓶插早期没有的谱带,在瓶插的第2阶段和第3阶段出现.到第4阶段和第5阶段又消失.如90.90～118.45 ku间的谱带、90.47～66.10 ku间的谱带、45.47 ku的谱带、42.81～29.75 ku间的谱带、24.93 ku的谱带、19.85 ku的谱带、16.74 ku的谱带等;而52.93、30.11 ku的谱带后期出现.可能这些蛋白的变化与衰老有关.3个不同品种洋桔梗其蛋白组分变化差异不大.
以3箇品種洋桔梗為材料.研究洋桔梗切花花瓣衰老過程中.可溶性蛋白質的變化情況.結果錶明,1-MCP(1-Methvlcyclopropene.1-甲基環丙烯)在延緩切花衰老,提高觀賞品質方麵有良好的作用;ETP(乙烯利)促進瞭蛋白質的分解,縮短瞭瓶插壽命.切花衰老過程中,蛋白質的組分和含量都髮生變化,有些蛋白質始終保持不變.如69.31、49.35、42.82、29.75 ku四條譜帶是始終存在;大部分蛋白質分解消失,如98.90、55.66、37.53、27.85、25.63、23.62、19.85、16.74 ku等譜帶到瓶插後期基本消失;瓶插早期沒有的譜帶,在瓶插的第2階段和第3階段齣現.到第4階段和第5階段又消失.如90.90～118.45 ku間的譜帶、90.47～66.10 ku間的譜帶、45.47 ku的譜帶、42.81～29.75 ku間的譜帶、24.93 ku的譜帶、19.85 ku的譜帶、16.74 ku的譜帶等;而52.93、30.11 ku的譜帶後期齣現.可能這些蛋白的變化與衰老有關.3箇不同品種洋桔梗其蛋白組分變化差異不大.
이3개품충양길경위재료.연구양길경절화화판쇠로과정중.가용성단백질적변화정황.결과표명,1-MCP(1-Methvlcyclopropene.1-갑기배병희)재연완절화쇠로,제고관상품질방면유량호적작용;ETP(을희리)촉진료단백질적분해,축단료병삽수명.절화쇠로과정중,단백질적조분화함량도발생변화,유사단백질시종보지불변.여69.31、49.35、42.82、29.75 ku사조보대시시종존재;대부분단백질분해소실,여98.90、55.66、37.53、27.85、25.63、23.62、19.85、16.74 ku등보대도병삽후기기본소실;병삽조기몰유적보대,재병삽적제2계단화제3계단출현.도제4계단화제5계단우소실.여90.90～118.45 ku간적보대、90.47～66.10 ku간적보대、45.47 ku적보대、42.81～29.75 ku간적보대、24.93 ku적보대、19.85 ku적보대、16.74 ku적보대등;이52.93、30.11 ku적보대후기출현.가능저사단백적변화여쇠로유관.3개불동품충양길경기단백조분변화차이불대.
Cut flowers of three cultivars of Eustoma grandiflorum were used as materials and detected in terms of proteins of the cut flowers during senescence. The result showed that 1-MCP(1-Methylcyclop ropene) had a good effect on delay in senescence of the cut flowers,which improved its ornamental value. ETP(ethrel) speeded up decomposition of the proteins of the cut flowers and shortened their vase life. During the process of senescence of the cut flowers,protein components and their content were changed; some proteins had no change,for example,four bands(69.31,49.35,42.82,29.75 ku) remained existing throughout the senescence; most proteins disappeared after decomposed,for example,the bands 98.90,55.66,37.53,27.85,25.63,23.62,19.85,16.74 ku which almost disappeared at the end of the vase stage; some bands were not observed at the early vase stage,but occurred at the vase stages Ⅱ and Ⅲ,and then disappeared at the vase stages Ⅳ and Ⅴ,e.g.,for example,proteins at 90.90-118.45 ku,90.47～66.10 ku,45.47 ku,42.81～29.75 ku,24.93 ku,19.85 ku,and 16.74 ku. Some proteins appeared at the late stage of senescence,for example,bands of 52.93 ku and 30.11 ku,which might have some relation with senescence. The change of protein components of the cut flowers was not apparently different among the 3 cultivars of E. grandiflorum.
