CAJ | 학술논문

采用活性跟踪法从金鸡菊根中分离获得抗病毒活性物质,经质谱和核磁共振分析,鉴定该物质为1-苯基-1,3,5-三庚炔.采用半叶枯斑法、叶圆盘法测定了该物质对烟草花叶病毒的抑制效果,结果表明,0.2 mg/mL的该化合物对TMV表现出较好的体外抑制侵染和增殖活性,其对TMV侵染和复制的抑制率分别为73.5%和84.3%.实时荧光定量PCR测定结果表明,该化合物对TMV外壳蛋白基因的表达有明显的抑制作用,0.2 mg/mL的该化合物对TMV外壳蛋白基因表达的抑制率为79.8%.图6表1参19
채용활성근종법종금계국근중분리획득항병독활성물질,경질보화핵자공진분석,감정해물질위1-분기-1,3,5-삼경결.채용반협고반법、협원반법측정료해물질대연초화협병독적억제효과,결과표명,0.2 mg/mL적해화합물대TMV표현출교호적체외억제침염화증식활성,기대TMV침염화복제적억제솔분별위73.5%화84.3%.실시형광정량PCR측정결과표명,해화합물대TMV외각단백기인적표체유명현적억제작용,0.2 mg/mL적해화합물대TMV외각단백기인표체적억제솔위79.8%.도6표1삼19
Production of plant secondary metabolites as a rich source of antimicrobial agents has been extensively applied in Chinese traditional medicine to treat viral diseases. Therefore, direct selection of antiphytoviral compounds from plants can be used to identify new potent antiviral agents. To screen antiviral compounds, tobacco mosaic virus (TMV) was chosen as a model target and the extracts with anti-TMV activity from 108 species of plants were analyzed. Among them, a extract from Coreopsis drummondii, predominantly suppressing the infection and replication of TMV was selected, and a constituent with anti-virus activity was isolated from it by bioassay. The compound was identified as 1-phenyl-l,3,5-triheptalkyne using MS and NMR. Its activity against TMV was investigated by local lesion and leaf discs assay. The results indicated that the compound showed a significant inhibitory activity against TMV in vitro, with 73.5% inhibitory rate against the infection of TMV and 84.3% against replication of TMV at a concentration of 0.2 mg/mL, while its inhibitory effect on TMV-CP gene expression in tobacco leaf discs was examined by real-time quantitative PCR, which showed that the compound obviously suppressed the expression of TMV-CP gene, with 79.8% inhibitory rate at the concentration of 0.2 mg/mL. Taken together, the anti-TMV activity of the compound contributes itself as a new potential candidate for development of antiviral drugs. Fig 6, Tab 1, Ref 19