CAJ | 학술논문

本试验选用唐古特白刺幼嫩茎段和叶片作为材料,研究白刺不同外植体的离体培养技术及其适宜的培养基.结果表明,白刺带芽嫩茎是诱导丛生芽的良好外植体,恒温20℃以下,可有效降低白刺丛生芽初代培养污染严重的程度;叶片是诱导愈伤组织的良好外植体,且低浓度生长素2,4-D培养基较高浓度培养基形成的白刺愈伤组织致密,也不易褐化.白刺的最适增殖、壮芽培养基为MS+6-BA 2 mg/L+NAA 1.00 mg/L,而且是以腋芽形成丛生芽的方式进行增殖的;最适生根培养基是1/2 MS+KT 1 mg/L+IBA 0.5 mg/L;形成愈伤组织较好的培养基是MS+2,4-D 0.5～1.0 mg/L.
본시험선용당고특백자유눈경단화협편작위재료,연구백자불동외식체적리체배양기술급기괄의적배양기.결과표명,백자대아눈경시유도총생아적량호외식체,항온20℃이하,가유효강저백자총생아초대배양오염엄중적정도;협편시유도유상조직적량호외식체,차저농도생장소2,4-D배양기교고농도배양기형성적백자유상조직치밀,야불역갈화.백자적최괄증식、장아배양기위MS+6-BA 2 mg/L+NAA 1.00 mg/L,이차시이액아형성총생아적방식진행증식적;최괄생근배양기시1/2 MS+KT 1 mg/L+IBA 0.5 mg/L;형성유상조직교호적배양기시MS+2,4-D 0.5～1.0 mg/L.
Young stem segments and leaves of Nitraria tangutorum were selected to investigate suitable media and plant regeneration techniques for explants. Immature stems were a good source for explants of tufted shoots, and cultivation at 20℃ can reduce contamination rates of tufted shoots. Leaves were good explants of callus induction as well and a lower concentration of 2,4-D was better for callus than a higher concentration. The optimum multiplication medium for N. tangutorum was MS+6-BA 2 mg/L+NAA 1.00 mg/L and tufted shoots formed from axil buds were the main multiplication method. The optimum medium for root formation was 1/2 MS+KT 1 mg/L+IBA 0.5 mg/L and the optimum medium for callus induction was MS+2.4-D 0.5-1.0 mg/L.