CAJ | 학술논문

黄足黑守瓜是普通丝瓜生长过程中危害叶片的主要害虫.本文对普通丝瓜不同部位叶片挥发性成分、营养成分和物理结构对黄足黑守瓜取食和定位的影响进行了系统研究.本实验采用顶空固相微萃取-气相色谱-质谱法分析了普通丝瓜不同部位叶片挥发性成分,研究结果表明,嫩叶主要挥发性成分有(Z)-3-己烯-1-醇(27.65%)和植醇(45.77%),成熟叶挥发性成分主要有(Z)-3-己烯-1-醇(49.34%),老叶挥发性成分主要有植醇(63.25%),且挥发性成分对黄足黑守瓜远距离定位起到了引导作用.同时,本研究还采用5点取样法、3,5-二硝基水杨酸法和考马斯亮蓝法分别研究了普通丝瓜不同部位叶片营养成分含量与黄足黑守瓜取食之间的关系,实验结果表明,黄足黑守瓜喜食植物叶片嫩叶部位,喜食部位叶片的总糖与还原糖含量、蛋白质含量都最高,且总糖含量与蛋白质含量比值最高.叶片物理结构调查采用石蜡切片法,发现叶片的物理结构对于黄足黑守瓜取食很少造成影响.通过研究,使我们充分地了解影响黄足黑守瓜取食不同部位叶片的因素,便于今后的育种和栽培过程中加强不利于黄足黑守瓜取食的因素,以达到安全、环保地进行农业生产的目的.
황족흑수과시보통사과생장과정중위해협편적주요해충.본문대보통사과불동부위협편휘발성성분、영양성분화물리결구대황족흑수과취식화정위적영향진행료계통연구.본실험채용정공고상미췌취-기상색보-질보법분석료보통사과불동부위협편휘발성성분,연구결과표명,눈협주요휘발성성분유(Z)-3-기희-1-순(27.65%)화식순(45.77%),성숙협휘발성성분주요유(Z)-3-기희-1-순(49.34%),로협휘발성성분주요유식순(63.25%),차휘발성성분대황족흑수과원거리정위기도료인도작용.동시,본연구환채용5점취양법、3,5-이초기수양산법화고마사량람법분별연구료보통사과불동부위협편영양성분함량여황족흑수과취식지간적관계,실험결과표명,황족흑수과희식식물협편눈협부위,희식부위협편적총당여환원당함량、단백질함량도최고,차총당함량여단백질함량비치최고.협편물리결구조사채용석사절편법,발현협편적물리결구대우황족흑수과취식흔소조성영향.통과연구,사아문충분지료해영향황족흑수과취식불동부위협편적인소,편우금후적육충화재배과정중가강불리우황족흑수과취식적인소,이체도안전、배보지진행농업생산적목적.
Aulacophora lewisii is a kind of major insect pest endangering leaves during the development of towel gourd (Luffa cylindrica (L.) Roem).In this paper,we have conducted a systematic research about the effects of volatile components,nutrition compounds content and physical structure from different parts of towel gourd leaves on feeding and orientation of A.lewisii.We employed the headspace solid phase microextraction-gas chromatography-mass spectrometry to be as the experiment method for analyzing the volatile components in different parts of towel gourd leaves.The results showed that 27.65% of the main volatile compounds in young leaves was phytol and 45.77% of that was (Z)-3-hexen-l-ol,49.34% of the main volatile compounds in fully grown leaves was (Z)-3-hex-en-l-ol,and 63.25% of the main volatile compounds in withered leaves was phytol.And further analysis revealed that the volatile compounds had an inducing effect on the far-distance orientation of A.lewisii.At the same time,we analyzed the relationship between nutrition compounds content in different parts of towel gourd leaves and feeding degree of A.lewisii by using the method of five-point sampling,3,5-dinitrosalicylic acid colorimetry and coomassie brilliant blue approach,respectively.The results illusrtated that A.lewisii prefered to feed tender leaves,in which the content of revert sugar,total sugar and protein was the highest,and so was the ratio of the total sugar content and protein content.The investigation of physical structure of different parts of the leaves was also studied by the method of paraffin slice,which demonstrated that the physical structure had little effect on feeding of A.lewisii.According to the research,we understand sufficiently the factors affecting the feeding and orientation of A.lewisii,which will facilitate to strengthen the unfavorable factors for A.lewisii feeding in breeding and cultivation to achieve the goal that carrying out agricultural production safely and environment-friendly.