安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
36期
17981-17982
,共2页
泥鳅%精子活力%钾%钠%钙%镁
泥鰍%精子活力%鉀%鈉%鈣%鎂
니추%정자활력%갑%납%개%미
Misgurnus anguillicaudatus%Sperm activity%Potassium%Sodium%Calcium%Magnesium
[目的]探讨泥鳅精子在不同浓度K~+、Na~+、Ca~(2+)、Mg~(2+)溶液中的活力并确定泥鳅精子激活液的理化因子的最佳浓度参数.[方法]用挤压法采精,通过观察和比较泥鳅精子在不同试验溶液中的活力,研究了几种化学因子对泥鳅精子活力的影响.[结果]在KCl溶液浓度为0.5%时,其中的泥鳅精子活力达到最高,为246 s,此后随KCl溶液浓度的增大而陡降.在NaCl溶液浓度为0.4%时,其中的泥鳅精子活力达到最高,为100 s,低于精子在KCl溶液中的最高活力,此后随NaCl溶液浓度的增大而快速降低.在CaCl_2溶液浓度为0.4%时,其中的泥鳅精子活力达到最高,为27 s,在MgCl_2溶液浓度为0.3%时,其中的泥鳅精子活力达到最高,为20 s,此后精子活力随二者浓度的增加均表现出缓慢下降的趋势.[结论]在鱼类的人工授精过程中,应避免使用高浓度Ca~(2+)、Mg~(2+)水配制精子激活液.
[目的]探討泥鰍精子在不同濃度K~+、Na~+、Ca~(2+)、Mg~(2+)溶液中的活力併確定泥鰍精子激活液的理化因子的最佳濃度參數.[方法]用擠壓法採精,通過觀察和比較泥鰍精子在不同試驗溶液中的活力,研究瞭幾種化學因子對泥鰍精子活力的影響.[結果]在KCl溶液濃度為0.5%時,其中的泥鰍精子活力達到最高,為246 s,此後隨KCl溶液濃度的增大而陡降.在NaCl溶液濃度為0.4%時,其中的泥鰍精子活力達到最高,為100 s,低于精子在KCl溶液中的最高活力,此後隨NaCl溶液濃度的增大而快速降低.在CaCl_2溶液濃度為0.4%時,其中的泥鰍精子活力達到最高,為27 s,在MgCl_2溶液濃度為0.3%時,其中的泥鰍精子活力達到最高,為20 s,此後精子活力隨二者濃度的增加均錶現齣緩慢下降的趨勢.[結論]在魚類的人工授精過程中,應避免使用高濃度Ca~(2+)、Mg~(2+)水配製精子激活液.
[목적]탐토니추정자재불동농도K~+、Na~+、Ca~(2+)、Mg~(2+)용액중적활력병학정니추정자격활액적이화인자적최가농도삼수.[방법]용제압법채정,통과관찰화비교니추정자재불동시험용액중적활력,연구료궤충화학인자대니추정자활력적영향.[결과]재KCl용액농도위0.5%시,기중적니추정자활력체도최고,위246 s,차후수KCl용액농도적증대이두강.재NaCl용액농도위0.4%시,기중적니추정자활력체도최고,위100 s,저우정자재KCl용액중적최고활력,차후수NaCl용액농도적증대이쾌속강저.재CaCl_2용액농도위0.4%시,기중적니추정자활력체도최고,위27 s,재MgCl_2용액농도위0.3%시,기중적니추정자활력체도최고,위20 s,차후정자활력수이자농도적증가균표현출완만하강적추세.[결론]재어류적인공수정과정중,응피면사용고농도Ca~(2+)、Mg~(2+)수배제정자격활액.
[Objective] The aim was to discuss the activities of Misgurnus anguillicaudatus sperms in solutions of K~+, Na~+, Ca~(2+) and Mg~(2+) at different concn. and ascertain the optimum concn. parameters of physicochemical factors of activation solution for M. anguillicaudatus sperms. [Method] The sperms were collected by squeezing method and the effects of several chemical factors on the activity of M. anguillicaudatus sperms were studied through observing and comparing their activities in different tested solutions. [Result] The activity of M. anguillicaudatus sperms in KCl solution reached highest when its concn. was 0.5%, being 246 s and then it was decreased sharply along with the increase of the concn. The activity of M. anguillicaudatus sperms in NaCl solution reached highest when its concn. was 0.4%, being 100 s which was lower than the highest activity of sperms in KCl solution, and then it was decreased rapidly along with the increase of the concn. of NaCl solution. The activity of M. anguillicaudatus sperms in CaCl_2 solution reached highest when its concn. was 0.4%, being 27 s and that in MgCl_2 solution reached highest when its concn. was 0.3%, being 20 s, and then the sperm activities showed slow decreasing trend in both the two solutions along with the increase of their concn. [Conclusion] In the artificial fertilization process of fishes, the use of water with Ca~(2+) and Mg~(2+) at high concn. should be avoided in preparing activation solutions for sperms.
