CAJ | 학술논문

基于血红蛋白(Hb)对过氧化氢氧化靛蓝胭脂红体系的催化作用, 建立了模拟酶催化荧光光度法测定血红蛋白的新方法并对催化氧化反应的机理进行了初步探讨. 实验发现体系的荧光强度与血红蛋白浓度在6.20×10~(-11)～7.75×10~(-8) mol/L范围内线性关系良好, 方法的检出限为1.67×10~(-11) mol/L. 方法用于人血中血红蛋白含量的测定, 回收率为98.8%～103.2%.
기우혈홍단백(Hb)대과양화경양화전람연지홍체계적최화작용, 건립료모의매최화형광광도법측정혈홍단백적신방법병대최화양화반응적궤리진행료초보탐토. 실험발현체계적형광강도여혈홍단백농도재6.20×10~(-11)～7.75×10~(-8) mol/L범위내선성관계량호, 방법적검출한위1.67×10~(-11) mol/L. 방법용우인혈중혈홍단백함량적측정, 회수솔위98.8%～103.2%.
A new spectroflurometric method was developed for the determination of hemoglobin based on its catalytic activity of mimetic peroxidase on oxidation of indigo carmine in the presence of hydrogen peroxide. The mechanism of the catalytic reaction was studied briefly. The kinetic parameter of reaction and the optimum condition were discussed and the results indicated that hemoglobin concentration demonstrated good linear relationship in the range of 6.2×10~(-11)～7.75×10~(-8) mol/L with a detection limit of 1.67×10~(-11) mol/L. The method has been applied to determination of the hemoglobin concentration in human blood samples. The recoveries were in the range from 98.8% to 103.2%.