CAJ | 학술논문

建立了高效液相色谱二极管阵列检测(HPLC-DAD)法同时测定丹参滴注液中丹参素、原儿茶醛、迷迭香酸和丹酚酸B四种水溶性成分的含量.采用Diamonsil~(TM)C_(18)色谱柱(250×4.6 mm,5 μm),以甲醇和5%冰乙酸为流动相进行梯度洗脱,流速为1.0 mL/min,柱温30℃,检测波长为286 nm.在此色谱条件下四种水溶性成分可完全分离.丹参素、原儿茶醛、迷迭香酸和丹酚酸B的线性范围分别为0.2192～1.934 μg(r=0.9999),0.03508～0.2456 μg(r=1.0000),0.2592～1.814 μg(r=1.0000),0.3864～2.705 μg(r=0.9999).平均回收率丹参素为102.6%,相对标准偏差(RSD)为0.55%;原儿茶醛为103.5%,RSD为0.42%;迷迭香酸为99.8%,RSD为0.68%;丹酚酸B为102.8%,RSD为0.49%.该方法简单、快速,四组分分离良好,可用于丹参滴注液的质量控制.
건립료고효액상색보이겁관진렬검측(HPLC-DAD)법동시측정단삼적주액중단삼소、원인다철、미질향산화단분산B사충수용성성분적함량.채용Diamonsil~(TM)C_(18)색보주(250×4.6 mm,5 μm),이갑순화5%빙을산위류동상진행제도세탈,류속위1.0 mL/min,주온30℃,검측파장위286 nm.재차색보조건하사충수용성성분가완전분리.단삼소、원인다철、미질향산화단분산B적선성범위분별위0.2192～1.934 μg(r=0.9999),0.03508～0.2456 μg(r=1.0000),0.2592～1.814 μg(r=1.0000),0.3864～2.705 μg(r=0.9999).평균회수솔단삼소위102.6%,상대표준편차(RSD)위0.55%;원인다철위103.5%,RSD위0.42%;미질향산위99.8%,RSD위0.68%;단분산B위102.8%,RSD위0.49%.해방법간단、쾌속,사조분분리량호,가용우단삼적주액적질량공제.
A high performance liquid chromatographic method with diode array detection was established for simultaneous determination of 4 water-soluble compounds of danshensu, protocatechuic aldehyde,rosmarinic acid and salvianolic aicd B in danshen dripping solution.The column used was Diamonsil~(TM)C_(18)(250×4.6 mm,5 μm). The mobile phase was methanol and 5% glacial acetic acid with gradient elution and the flow rate was 1.0ml/min, the column temperature was 30℃, and the detection wavelength was 286 nm. The linearity was obtained over the range of 0.2192～1.934 μg(r=0.9999)for danshensu with average recovery of 102.6%(RSD=0.55%), 0.03508～0.2456 μg(r=1.0000)for protocatechuic aldehyde with average recovery of 103.5%(RSD=0.42%),0.2592～1.814 μg(r=1.0000)for rosmarinic acid with average recovery of 99.8%(RSD=0.68%), 0.3864～2.705 μg(r=0.9999)for salvianolic aicd B with average recovery of 102.8%(RSD=0.49%), respectively. The method is rapid, accurate and reliable, and can be used to control the quality of Danshen dripping solution.