南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2010年
3期
463-467
,共5页
头孢噻肟%T-RFIP%底泥%微生物群落结构%bla_(CTX-M)
頭孢噻肟%T-RFIP%底泥%微生物群落結構%bla_(CTX-M)
두포새우%T-RFIP%저니%미생물군락결구%bla_(CTX-M)
cefotaxime%terminal restriction fragment length polymorphism%sediment%microbial community%bla_(CTX-M)
目的 研究抗生素(头孢噻肟)作用下河流沉积物微生物群落结构的变化及耐药基因bla_(CTX-M)多样性.方法 应用基于16S rRNA的末端限制性酶切片段长度多态性(T-RFLP)技术,分析分别在0、6.4、64和320 mg的头孢噻肟作用下,城市河流底泥细菌群落结构变化;并通过巢式PCR,分析其耐药基因bla_(CTX-M)的多样性变化.结果 T-RF特征峰统计分析表明:不同抗生素浓度处理,与底泥细菌群落结构多样性变化之间无显著相关性;但实验室处理时间会导致群落结构产生显著变化.PCR结果 表明:随实验室处理时间的延长,耐药基因bla_(CTX-M)多样性下降.结论 对已承受排放废水污染的河流沉积物,由于其中富含大量的耐药菌群,其总体菌群结构对抗生素的作用不敏感.同时,对非原位开展的菌群结构研究,实验室处理时间是重要的影响因素.
目的 研究抗生素(頭孢噻肟)作用下河流沉積物微生物群落結構的變化及耐藥基因bla_(CTX-M)多樣性.方法 應用基于16S rRNA的末耑限製性酶切片段長度多態性(T-RFLP)技術,分析分彆在0、6.4、64和320 mg的頭孢噻肟作用下,城市河流底泥細菌群落結構變化;併通過巢式PCR,分析其耐藥基因bla_(CTX-M)的多樣性變化.結果 T-RF特徵峰統計分析錶明:不同抗生素濃度處理,與底泥細菌群落結構多樣性變化之間無顯著相關性;但實驗室處理時間會導緻群落結構產生顯著變化.PCR結果 錶明:隨實驗室處理時間的延長,耐藥基因bla_(CTX-M)多樣性下降.結論 對已承受排放廢水汙染的河流沉積物,由于其中富含大量的耐藥菌群,其總體菌群結構對抗生素的作用不敏感.同時,對非原位開展的菌群結構研究,實驗室處理時間是重要的影響因素.
목적 연구항생소(두포새우)작용하하류침적물미생물군락결구적변화급내약기인bla_(CTX-M)다양성.방법 응용기우16S rRNA적말단한제성매절편단장도다태성(T-RFLP)기술,분석분별재0、6.4、64화320 mg적두포새우작용하,성시하류저니세균군락결구변화;병통과소식PCR,분석기내약기인bla_(CTX-M)적다양성변화.결과 T-RF특정봉통계분석표명:불동항생소농도처리,여저니세균군락결구다양성변화지간무현저상관성;단실험실처리시간회도치군락결구산생현저변화.PCR결과 표명:수실험실처리시간적연장,내약기인bla_(CTX-M)다양성하강.결론 대이승수배방폐수오염적하류침적물,유우기중부함대량적내약균군,기총체균군결구대항생소적작용불민감.동시,대비원위개전적균군결구연구,실험실처리시간시중요적영향인소.
Objective To explore the diversity changes of microbial communities and bla_(CTX-M) in an urban river sediment community with cefotaxime treatment. Methods The terminal restriction fragment length polymorphism (T-RFLP) of 16S rDNA was employed to analyze the bacteria community, and the bla_(CTX-M) was determined using nested PCR. The sediment was treated by cefotaxime at the concentrations of 0, 6.4, 64, and 320 mg/L. Results According to statistical analysis of the T-RFs, no significant correlation was observed between the antibiotic treatment and the microbial community change, but incubation in laboratory conditions had significant effect on the microbial diversity. The PCR results showed that the diversity of bla_(CTX-M) decreased after the laboratory incubation. Conclusion As there are diverse antibiotic resistant bacteria in the sediments with prior pollution by antibiotics, the whole community can be inert to the antibiotic treatment. Nevertheless, the ex situ treatment conditions in laboratory studies have a significant impact on the community structures.
